An easily-performed high-throughput method for plant genomic DNA extraction

被引：11

作者：

Yu, Deshui ^{[1
,2
]}

Zhang, Ju ^{[1
,2
]}

Tan, Guangxuan ^{[1
,2
]}

Yu, Ningshu ^{[1
,2
,3
]}

Wang, Qiuyue ^{[1
,2
,3
]}

Duan, Qiqi ^{[1
,2
,3
]}

Qi, Xini ^{[1
,2
,3
]}

Cheng, Mingjiao ^{[1
,2
,3
]}

Yan, Chunxue ^{[1
,2
,3
]}

Wei, Zhangkun ^{[1
,2
,3
]}

Yu, Zhenmiao ^{[4
]}

Huang, Wenchao ^{[4
]}

Li, Chengwei ^{[1
,2
,5
]}

机构：

[1] Zhoukou Normal Univ, Key Lab Plant Genet & Mol Breeding, Zhoukou 466001, Peoples R China

[2] Henan Key Lab Crop Mol Breeding & Bioreactor, Zhoukou 466001, Peoples R China

[3] Zhoukou Normal Univ, Coll Life Sci & Agron, Zhoukou 466001, Peoples R China

[4] Dancheng 1 Senior High Sch, Dancheng 477150, Zhoukou, Peoples R China

[5] Henan Inst Sci & Technol, Henan Engn Res Ctr Crop Genome Editing, Xinxiang 453003, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2019年 / 569卷

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/j.ab.2019.01.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic DNA isolation is a crucial technique for researchers studying plant molecular biology. A current widely-used protocol for DNA extraction needs a pestle and mortal for each sample and consumes a large amount of liquid nitrogen in grinding the samples. Most high-throughput methods depend on expensive machines for tissue homogenization. Here we developed a CTAB-based DNA extraction method using 2.0 mL microcentrifuge tubes for sample processing. This protocol has the advantages that it is suitable for a variety of plants, easily-performed without special equipment, and high-throughput; it effectively avoids sample cross-contamination, and is inexpensive, rapid and safe.

引用

页码：28 / 30

页数：3

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