Formation of actin stress fibers and focal adhesions enhanced by Rho-kinase

被引:931
|
作者
Amano, M
Chihara, K
Kimura, K
Fukata, Y
Nakamura, N
Matsuura, Y
Kaibuchi, K
机构
[1] NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT,IKOMA 63001,JAPAN
[2] KYOTO UNIV,FAC MED,DEPT ANAT 2,KYOTO 606,JAPAN
[3] NATL INST HLTH,DEPT VIROL 2,TOKYO 162,JAPAN
关键词
D O I
10.1126/science.275.5304.1308
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The small guanosine triphosphatase (GTPase) Rho is implicated in the formation of stress fibers and focal adhesions in fibroblasts stimulated by extracellular signals such as lysophosphatidic acid (LPA). Rho-kinase is activated by Rho and may mediate some biological effects of Rho. Microinjection of the catalytic domain of Rho-kinase into serum-starved Swiss 3T3 cells induced the formation of stress fibers and focal adhesions, whereas microinjection of the inactive catalytic domain, the Rho-binding domain, or the pleckstrin-homology domain inhibited the LPA-induced formation of stress fibers and focal adhesions. Thus, Rho-kinase appears to mediate signals from Rho and to induce the formation of stress fibers and focal adhesions.
引用
收藏
页码:1308 / 1311
页数:6
相关论文
共 50 条
  • [41] Enhanced Rho-kinase dependent contractility in fibroblast from COPD patients
    Hallgren, O.
    Nihlberg, K.
    Wieslander, E.
    Kvist-Reimer, M.
    Dahlback, M.
    Eriksson, L.
    Bjermer, L.
    Erjefalt, J.
    Lofdahl, C. -G.
    Westergren-Thorsson, G.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2010, 181
  • [42] Rho-kinase inhibitor upregulates migration by altering focal adhesion formation via the Akt pathway in colon cancer cells
    Adachi, Seiji
    Yasuda, Ichiro
    Nakashima, Masanori
    Yamauchi, Takahiro
    Yoshioka, Takashi
    Okano, Yukio
    Moriwaki, Hisataka
    Kozawa, Osamu
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2011, 650 (01) : 145 - 150
  • [43] Rho-Kinase Mediates ER Stress-Induced Endothelial Dysfunction
    Kawanami, Daiji
    Matoba, Keiichiro
    Okada, Rina
    Tsukamoto, Masami
    Kinoshita, Jun
    Ito, Tomoko
    Ishizawa, Sho
    Kanazawa, Yasushi
    Yokota, Tamotsu
    Utsunomiya, Kazunori
    DIABETES, 2012, 61 : A127 - A127
  • [44] Effect of Stress on the Expression of Rho-Kinase and Collagen in Rat Bladder Tissue
    Yoon, Hana
    Lee, Donghyun
    Chun, Kyemin
    Yoon, Hyunsuk
    Yoo, Jaeyeong
    KOREAN JOURNAL OF UROLOGY, 2010, 51 (02) : 132 - 138
  • [45] Dynamic interplay between filopodia, focal adhesions and stress fibers.
    Young, L. E.
    Higgs, H. N.
    MOLECULAR BIOLOGY OF THE CELL, 2017, 28
  • [46] FOCAL ADHESIONS, STRESS FIBERS AND EXTRACELLULAR-MATRIX IN RPE CELLS
    OPAS, M
    KALNINS, VI
    ANATOMICAL RECORD, 1985, 211 (03): : A144 - A144
  • [47] Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed
    Gardel, Margaret L.
    Sabass, Benedikt
    Ji, Lin
    Danuser, Gaudenz
    Schwarz, Ulrich S.
    Waterman, Clare M.
    JOURNAL OF CELL BIOLOGY, 2008, 183 (06): : 999 - 1005
  • [48] Activation of the Rho/Rho-kinase pathway in the brainstem is involved in enhanced sympathetic drive in mice with myocardial infarction
    Kimura, Y
    Hirooka, Y
    Sagara, Y
    Nozoe, M
    Sunagawa, K
    FASEB JOURNAL, 2005, 19 (05): : A1595 - A1595
  • [49] Rho-kinase activity contributes to cerebral vascular tone and is enhanced during hypertension
    Sobey, CG
    Chrissobolis, S
    FASEB JOURNAL, 2001, 15 (04): : A124 - A124
  • [50] Endothelin-1 Induction of Focal Adhesion Kinase in Normal Human Lung Fibroblasts is Dependent on Rho-kinase
    Kulasekaran, P.
    Thannickal, V. J.
    Horowitz, J. C.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2010, 181