Use of a one atmosphere uniform glow discharge plasma to kill a broad spectrum of microorganisms

The medical, industrial, and food processing industries are constantly in search of new technologies to provide improved methods of sterilization and pasteurization. Proposed techniques must deal with such problems as thermal sensitivity and destruction by heat, formation of toxic by-products, cost, and inefficiency in performance. We report results from a newly invented plasma source, a one atmosphere uniform glow discharge plasma (OAUGDP), which is capable of operating at atmospheric pressure in air and providing antimicrobial active species at room temperature. OAUGDP exposures have reduced log numbers of bacteria (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa), bacterial endospores (Bacillus subtilis and Bacillus pumilus), and various yeast and bacterial viruses on a variety of surfaces. These surfaces included polypropylene, filter paper, paper strips, solid culture media, and glass. Experimental results showed at least a greater than or equal to 5 log(10) colony forming units (CFU) reduction in bacteria within a range of 15-90 s of exposure, whether the samples were exposed in conventional sterilization bags or exposed directly to the plasma. An exception to these very short exposure times were experiments with solid culture media in which 5 min of plasma exposure was necessary to produce greater than or equal to 5 log(10) CFU reduction in bacterial counts. The effects of plasma treatment on bacterial cell structures were investigated by exposing cells to plasma for various durations and examining them by transmission electron microscopy. The results showed that cell breakage (lysis) occurred with the release of cellular contents. These data were consistent with spectrophotometric results in: which the release of cellular constituents was measured as a change in ultraviolet absorption at 260 nm. With all microorganisms tested, a biphasic survival curve (logarithmic number of survivors versus time) was observed in plots of dose-response data. Differences in susceptibilities of microorganisms observed on various surfaces suggested that the degree of lethality was dependent upon the time of diffusion of active species through the medium to the organism and the makeup of the microbial cellular surface. (C) 1999 American Vacuum Society. [S0734-2101(99)09904-2].