Method for preparing tritium-labeled S-adenosyl-L-methionine using methionine-adenosyltransferase from rabbit liver

被引：0

作者：

Lazurkina, TY ^{[1
]}

Shram, SI ^{[1
]}

Rybakova, IG ^{[1
]}

Myasoedov, NF ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Genet Mol, Moscow, Russia

来源：

RADIOCHEMISTRY | 1999年 / 41卷 / 04期

关键词：

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and efficient procedure was proposed for preparing S-adenosyl-L-methionine tritium-labeled at the methyl group using partially purified methionine-adenosyltransferase from rabbit liver. In a preparative synthesis, from 6 mCi of L-[H-3]methionine was obtained 3.3 mCi of S-adenosyl-L- [H-3]methionine with a 98% purity and a molar radioactivity of 20 Ci mmol(-1). The product was purified in a single stage by chromatograghy in a microcolumn packed with Amberlite CG-50.

引用

页码：376 / 379

页数：4

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