Detection of infectious bursal disease virus in clinical samples by dot-blot hybridization using a non radioactive DIG-labelled probe

被引：0

作者：

Kataria, RS ^{[1
]}

Tiwari, AK ^{[1
]}

Butchaiah, G ^{[1
]}

Prasad, N ^{[1
]}

Das, SK ^{[1
]}

机构：

[1] Indian Vet Res Inst, Natl Biotechnol Ctr, Izatnagar 243122, Uttar Pradesh, India

来源：

INDIAN VETERINARY JOURNAL | 2004年 / 81卷 / 01期

关键词：

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Infectious bursal disease (IBD) is a highly contagious immuno-suppressive viral disease of young chickens: Out of the two serotypes of IBD virus (IBDV) reported, only serotype1 viruses are pathogenic to chickens whereas, serotype2 viruses are apathogenic in nature. In India, after 1992 emergence of very virulent IBD viruses resulted in more than 70% mortality. We report here use of DIG-labelled probe prepared from RT-PCR amplified 552 bpVP2 gene sequence for the detection of IBDV in clinical samples.

引用

页码：90 / 91

页数：2

共 36 条

[31] Rapid detection of infectious bursal disease virus using one-step RT-PCR in clinical samples in Pakistan
Zahoor, M. A.
Hussain, I.
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JOURNAL OF DAIRY SCIENCE, 2004, 87 : 109 - 109
[32] DETECTION OF CYTOMEGALO-VIRUS BY DOT-BLOT DNA HYBRIDIZATION USING PROBES LABELED WITH P-32 BY NICK TRANSLATION OR RANDOM HEXANUCLEOTIDE PRIMING
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[33] A comparison of four DNA extraction methods for the detection of Citrus yellow mosaic badna virus from two species of citrus using PCR and dot-blot hybridization
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[34] Direct detection of infectious bursal disease virus from clinical samples by in situ reverse transcriptase-linked polymerase chain reaction
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[35] SENSITIVE NON-RADIOACTIVE DOT BLOT HYBRIDIZATION USING DNA PROBES LABELED WITH CHELATE GROUP SUBSTITUTED PSORALEN AND QUANTITATIVE DETECTION BY EUROPIUM ION FLUORESCENCE
OSER, A
ROTH, WK
VALET, G
NUCLEIC ACIDS RESEARCH, 1988, 16 (03) : 1181 - 1196
[36] THE DETECTION OF HUMAN CYTOMEGALO-VIRUS MESSENGER-RNA IN CLINICAL SPECIMENS BY NON-RADIOACTIVE INSITU HYBRIDIZATION USING OLIGONUCLEOTIDE PROBES
COLLOBY, P
LIBETTA, C
PRINGLE, JH
DONNELLY, P
FLOWER, A
LAUDER, I
JOURNAL OF PATHOLOGY, 1989, 157 (02): : A163 - A163

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