Detection of infectious bursal disease virus in clinical samples by dot-blot hybridization using a non radioactive DIG-labelled probe

被引：0
|
作者：
Kataria, RS [1 ]
Tiwari, AK [1 ]
Butchaiah, G [1 ]
Prasad, N [1 ]
Das, SK [1 ]
机构：
[1] Indian Vet Res Inst, Natl Biotechnol Ctr, Izatnagar 243122, Uttar Pradesh, India
来源：
INDIAN VETERINARY JOURNAL | 2004年 / 81卷 / 01期
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中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Infectious bursal disease (IBD) is a highly contagious immuno-suppressive viral disease of young chickens: Out of the two serotypes of IBD virus (IBDV) reported, only serotype1 viruses are pathogenic to chickens whereas, serotype2 viruses are apathogenic in nature. In India, after 1992 emergence of very virulent IBD viruses resulted in more than 70% mortality. We report here use of DIG-labelled probe prepared from RT-PCR amplified 552 bpVP2 gene sequence for the detection of IBDV in clinical samples.
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页码：90 / 91
页数：2
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