Metabolomic and Transcriptomic Analyses of Escherichia coli for Efficient Fermentation of L-Fucose
被引:18
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作者:
Kim, Jungyeon
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机构:
Korea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South KoreaKorea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea
Kim, Jungyeon
[1
]
Cheong, Yu Eun
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机构:
Korea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South KoreaKorea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea
Cheong, Yu Eun
[1
]
Jung, Inho
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机构:
Korea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea
Korea Forestry Promot Inst, Forest Prod Anal & Certificat Div, Seoul 07570, South KoreaKorea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea
Jung, Inho
[1
,2
]
Kim, Kyoung Heon
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机构:
Korea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South KoreaKorea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea
Kim, Kyoung Heon
[1
]
机构:
[1] Korea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea
[2] Korea Forestry Promot Inst, Forest Prod Anal & Certificat Div, Seoul 07570, South Korea
fucose;
fermentation;
metabolomics;
transcriptomics;
Escherichia coli;
brown macroalgae;
SYSTEMS BIOLOGY;
CARBON SOURCE;
MACROALGAE;
BROWN;
ADAPTATION;
PLATFORM;
PATHWAY;
GROWTH;
D O I:
10.3390/md17020082
中图分类号:
R914 [药物化学];
学科分类号:
100701 ;
摘要:
L-Fucose, one of the major monomeric sugars in brown algae, possesses high potential for use in the large-scale production of bio-based products. Although fucose catabolic pathways have been enzymatically evaluated, the effects of fucose as a carbon source on intracellular metabolism in industrial microorganisms such as Escherichia coli are still not identified. To elucidate the effects of fucose on cellular metabolism and to find clues for efficient conversion of fucose into bio-based products, comparative metabolomic and transcriptomic analyses were performed on E. coli on L-fucose and on D-glucose as a control. When fucose was the carbon source for E. coli, integration of the two omics analyses revealed that excess gluconeogenesis and quorum sensing led to severe depletion of ATP, resulting in accumulation and export of fucose extracellularly. Therefore, metabolic engineering and optimization are needed for E. coil to more efficiently ferment fucose. This is the first multi-omics study investigating the effects of fucose on cellular metabolism in E. coli. These omics data and their biological interpretation could be used to assist metabolic engineering of E. coli producing bio-based products using fucose-containing brown macroalgae.