Aphididae;
COI;
DNA barcoding;
mitochondrial DNA;
parthenogenesis;
species identification;
D O I:
10.1111/j.1755-0998.2008.02297.x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
A 658-bp fragment of mitochondrial DNA from the 5 ' region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.
机构:
Univ Guam, Coll Nat & Appl Sci, Western Pacific Trop Res Ctr, Mangilao, GU 96923 USAUniv Guam, Coll Nat & Appl Sci, Western Pacific Trop Res Ctr, Mangilao, GU 96923 USA
Miller, Ross H.
Duay, Julie Anne M.
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机构:
Univ Guam, Coll Nat & Appl Sci, Western Pacific Trop Res Ctr, Mangilao, GU 96923 USAUniv Guam, Coll Nat & Appl Sci, Western Pacific Trop Res Ctr, Mangilao, GU 96923 USA
Duay, Julie Anne M.
Pike, Keith S.
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机构:
Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USAUniv Guam, Coll Nat & Appl Sci, Western Pacific Trop Res Ctr, Mangilao, GU 96923 USA
Pike, Keith S.
Maw, Eric
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机构:
Agr & Agri Food Canada, Canadian Natl Collect Insects, Ottawa, ON K1A 0C6, CanadaUniv Guam, Coll Nat & Appl Sci, Western Pacific Trop Res Ctr, Mangilao, GU 96923 USA
Maw, Eric
Foottit, Robert G.
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h-index: 0
机构:
Agr & Agri Food Canada, Canadian Natl Collect Insects, Ottawa, ON K1A 0C6, CanadaUniv Guam, Coll Nat & Appl Sci, Western Pacific Trop Res Ctr, Mangilao, GU 96923 USA