Aphididae;
COI;
DNA barcoding;
mitochondrial DNA;
parthenogenesis;
species identification;
D O I:
10.1111/j.1755-0998.2008.02297.x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
A 658-bp fragment of mitochondrial DNA from the 5 ' region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.
机构:
Int Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, Kenya
Kenyatta Univ, POB 43844-00100 GPO, Nairobi, KenyaInt Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, Kenya
Khamis, F. M.
Mohamed, S.
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机构:
Int Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, KenyaInt Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, Kenya
Mohamed, S.
Ombura, L. O.
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机构:
Int Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, KenyaInt Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, Kenya
Ombura, L. O.
Warigia, M.
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机构:
Kenyatta Univ, POB 43844-00100 GPO, Nairobi, KenyaInt Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, Kenya
Warigia, M.
Ekesi, S.
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机构:
Int Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, KenyaInt Ctr Insect Physiol & Ecol, POB 30772-00100 GPO, Nairobi, Kenya