Biofilm-formation by drug-resistant Staphylococcus aureus from cow milk

The present study aims to quantify biofilm formation and determine the drug resistance profile of Staphylococcus aureus isolated from raw, pasteurized and ultra-high temperature (UHT) milk Detection of S. aureus was made in 90 cow milk samples (30 raw, 30 pasteurized and 30 UHT). Biofilm biomass quantification was performed in 96-well polystyrene plates using the crystal violet technique. The resistance profile was determined by the disc-diffusion test. S. aureus was detected in 24.4% (22/90) of milk samples, with the highest prevalence in pasteurized milk Biofilm production was observed in 100% of the S. aureus isolates from pasteurized and UHT milk, and in 81.8% of the strains from raw milk Biofilm production by S. aureus drug-resistant strains was detected in 46.7% (14/30) of the isolates. In order to mitigate this problem, it is recommended to apply appropriate hygiene practices along the milk production chain.