Layered double hydroxide-galactose as an excellent nanocarrier for targeted delivery of curcumin to hepatocellular carcinoma cells

被引:30
|
作者
Mokhtari, Sarina [1 ]
Solati-Hashjin, Mehran [1 ]
Khosrowpour, Zahra [2 ,3 ]
Gholipourmalekabadi, Mazaher [2 ,3 ]
机构
[1] Amirkabir Univ Technol, Tehran Polytech, Dept Biomed Engn, BioFabricat Lab BFL, Tehran, Iran
[2] Iran Univ Med Sci, Cellular & Mol Res Ctr, Tehran, Iran
[3] Iran Univ Med Sci, Fac Adv Technol Med, Dept Tissue Engn & Regenerat Med, Shahid Hemmat Highway, Tehran 1449614535, Iran
关键词
Layered double hydroxides; Curcumin; Galactose; Targeted drug delivery systems; Hepatocellular carcinoma; IN-VITRO; SUSTAINED-RELEASE; NANOPARTICLES; CHEMOTHERAPY; ACID; NANOHYBRIDS; SCAFFOLDS; MICELLES; EFFICACY; THERAPY;
D O I
10.1016/j.clay.2020.105891
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Targeted drug delivery systems have been developed as a promising strategy for targeted delivery of the drugs, genes, and biomolecules to the target tissue. In this study, a drug delivery system based on layered double hydroxide (LDH), as a carrier, and galactose (Gal), as a ligand, was designed for targeted delivery of curcumin (Cur) to hepatocellular carcinoma cells. Gal was conjugated onto Cur/LDH nanohybrid using SiO2 nanodot-coating and amine-functionalization strategy to fabricate a Gal-Cur/LDH delivery system. The physicochemical properties were determined using field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM), zeta potential, Fourier transform infrared spectrometry (FTIR) and X-ray powder diffraction (XRD). The cell viability, cellular uptake, and apoptotic effects of the Gal-Cur/LDH nanoparticles for human hepatocellular carcinoma cell line (HepG2 cells) were evaluated. Additionally, in the cell viability test, to investigate the specific targeting efficiency of Gal-Cur/LDH nanoparticles into hepatoma cells, Murine fibmsarcoma L929 cells were utilized as control cells for studying the specific targeting efficiency of Gal-Cur/LDH nanoparticles for HepG2 cells. Results demonstrated the successful synthesis of LDH and the intercalation of Cur in LDH with 31.0 +/- 0.0% loading efficiency. According to the cyto assay results, LDH did not significantly induce cytotoxicity in both L929 and HepG2 cells. Gal-Cur/LDH nanoparticles showed higher cytotoxicity effects against HepG2 cells than L929 cells, compared with free Cur and Cur/LDH nanohybrid. Additionally, Gal-Cur/LDH nanoparticles significantly exhibited a higher rate of apoptosis than the other groups, which might be attributed to higher cellular uptake efficiency via ASGP-receptors in HepG2 cells surface. Our findings suggest the Gal-Cur/LDH nanoparticles as an excellent carrier system for targeted delivery of Cur to the hepatocellular carcinoma cells.
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页数:11
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