A panel of polymorphic EST-derived microsatellite loci for the small yellow croaker (Larimichthys polyactis)

被引：11
|
作者：
Wang, Xiuli [1 ]
Li, Depeng [1 ]
Meng, Xiangying [1 ]
Qiu, Xuemei [1 ]
机构：
[1] Dalian Fisheries Univ, Coll Life Sci & Biotechnol, Dalian 116023, Peoples R China
来源：
CONSERVATION GENETICS | 2009年 / 10卷 / 05期
关键词：
EST; SSRs; Small yellow croaker; Larimichthys polyactis; DNA;
D O I：
10.1007/s10592-009-9816-1
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Small yellow croaker (Larimichthys polyactis) is an important economic species of marine fishery. We developed and evaluated simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) of Pseudosciaena crocea, Paralichthys olivaceus and Psetta maxima. Characteristics of nine EST-SSR loci were investigated using 46 L. polyactis individuals. The number of alleles per locus ranged from 2 to 6. The observed heterozygosity ranged from 0.0652 to 0.7391, while the expected heterozygosity ranged from 0.0638 to 0.7754. Seven loci departed from Hardy-Weinberg equilibrium (P < 0.01) significantly. These loci and markers will be useful for population genetics and systemic evolution among species of small yellow croaker.
引用
收藏
页码：1629 / 1631
页数：3
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