Improved detection of Shigella using Escherichia coli medium enrichment: Polymerase chain reaction from stool samples

被引：5
|
作者：
Gupta, P. K. [1 ]
Appannanavar, S. B. [2 ]
Mohan, B. [3 ]
Taneja, N. [3 ]
机构：
[1] Dr RPGMC, Dept Microbiol, Kangra, Himachal Prades, India
[2] SDM Med Coll, Dept Microbiol, Dharwad, Karnataka, India
[3] Post Grad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh, India
来源：
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY | 2016年 / 34卷 / 04期
关键词：
Escherichia coli medium; polymerase chain reaction; Shigella; PCR; INFECTION; DIAGNOSIS; DIARRHEA; FLEXNERI;
D O I：
10.4103/0255-0857.195355
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Laboratory diagnosis of shigellosis using conventional culture technique is limited by lower sensitivity and higher turnaround time. Here, we have evaluated the role of polymerase chain reaction from stool samples after enrichment in Escherichia coli medium for detection of Shigellae. The technique not only increased the sensitivity but also decreased the turnaround time.
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页码：526 / 528
页数：3
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