Improved detection of Shigella using Escherichia coli medium enrichment: Polymerase chain reaction from stool samples

被引：5

作者：

Gupta, P. K. ^{[1
]}

Appannanavar, S. B. ^{[2
]}

Mohan, B. ^{[3
]}

Taneja, N. ^{[3
]}

机构：

[1] Dr RPGMC, Dept Microbiol, Kangra, Himachal Prades, India

[2] SDM Med Coll, Dept Microbiol, Dharwad, Karnataka, India

[3] Post Grad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh, India

来源：

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY | 2016年 / 34卷 / 04期

关键词：

Escherichia coli medium; polymerase chain reaction; Shigella; PCR; INFECTION; DIAGNOSIS; DIARRHEA; FLEXNERI;

D O I：

10.4103/0255-0857.195355

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Laboratory diagnosis of shigellosis using conventional culture technique is limited by lower sensitivity and higher turnaround time. Here, we have evaluated the role of polymerase chain reaction from stool samples after enrichment in Escherichia coli medium for detection of Shigellae. The technique not only increased the sensitivity but also decreased the turnaround time.

引用

页码：526 / 528

页数：3

共 50 条

[1] Detection of enterotoxigenic Escherichia coli in stool specimens by polymerase chain reaction
Yavzori, M
Porath, N
Ochana, O
Dagan, R
Orni-Wasserlauf, R
Cohen, D
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1998, 31 (04) : 503 - 509
[2] DETECTION OF SHIGELLA AND ENTEROINVASIVE ESCHERICHIA-COLI USING POLYMERASE CHAIN-REACTION
YE, LY
LAN, FH
ZHU, ZY
CHEN, XM
YE, XL
JOURNAL OF DIARRHOEAL DISEASES RESEARCH, 1993, 11 (01): : 38 - 40
[3] POLYMERASE CHAIN-REACTION FOR THE SPECIFIC DETECTION OF ESCHERICHIA-COLI SHIGELLA
SPIERINGS, G
OCKHUIJSEN, C
HOFSTRA, H
TOMMASSEN, J
RESEARCH IN MICROBIOLOGY, 1993, 144 (07) : 557 - 564
[4] Screening for toxigenic Escherichia coli in stool samples of diarrhoeal patients by polymerase chain reaction
Nawaz, Benish
Ali, Anum
Syed, Muhammad Noman
Khan, Abdul Basit
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2014, 27 (05) : 1571 - 1574
[5] Detection of Escherichia coli in Food Samples Using Culture and Polymerase Chain Reaction Methods
Hariri, Sumyya
CUREUS JOURNAL OF MEDICAL SCIENCE, 2022, 14 (12)
[6] POLYMERASE CHAIN-REACTION FOR DETECTION OF ADENOVIRUSES IN STOOL SAMPLES
ALLARD, A
GIRONES, R
JUTO, P
WADELL, G
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (12) : 2659 - 2667
[7] DETECTION OF ESCHERICHIA-COLI AND SHIGELLA SPP IN WATER BY USING THE POLYMERASE CHAIN-REACTION AND GENE PROBES FOR UID
BEJ, AK
DICESARE, JL
HAFF, L
ATLAS, RM
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (04) : 1013 - 1017
[8] DETECTION OF ASTROVIRUSES FROM STOOL SAMPLES IN JAPAN USING REVERSE TRANSCRIPTION AND POLYMERASE CHAIN-REACTION AMPLIFICATION
SAITO, K
USHIJIMA, H
NISHIO, O
OSETO, MK
MOTOHIRO, H
UEDA, Y
TAKAGI, M
NAKAYA, S
ANDO, T
GLASS, R
ZAIMAN, K
MICROBIOLOGY AND IMMUNOLOGY, 1995, 39 (10) : 825 - 828
[9] Detection of the eaeA gene in Escherichia coli from chickens by polymerase chain reaction
Kilic, Ayse
Ertas, Hasan Basri
Muz, Adile
Ozbey, Goekben
Kalender, Hakan
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2007, 31 (04): : 215 - 218
[10] Multiplex polymerase chain reaction method discriminating Escherichia coli and Shigella sp.
Yamazaki, Y.
Fukasawa, A.
ARCHIVES OF MICROBIOLOGY, 2011, 193 (02) : 83 - 87

← 1 2 3 4 5 →