Characteristics of L-lactic acid production by Rhizopus oryzae mutant RL6041

被引:1
|
作者
Zhou, XH [1 ]
Ge, CM [1 ]
Yao, JM [1 ]
Pan, RR [1 ]
Yu, ZL [1 ]
机构
[1] Chinese Acad Sci, Key Lab Ion Beam Bioengn, Hefei, Anhui, Peoples R China
关键词
Ions implantation; L (+)-lactic acid; Rhizopus oryzae; fermentation;
D O I
10.1080/08905430500316409
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A high-yield mutant strain Rhizopus oryzae RL6041 induced by ions implantation was used in the present study. L (+)-lactic acid was generated by RL6041 grown on liquefied corn starch as a substrate. With optimal conditions (Liquefied corn starch 150g/L, (NH4)(2)SO4 2.0g/L, MgSO4 center dot 7H(2)O 1.0g/L, ZnSO4 center dot 7H(2)O2g/L, KH2PO4 0.2g/L, CaCO3 8%, medium size 20/250Ml, seed age 6h, fermentation temperature 38 C), the yield of L (+)-lactic acid in shake-flask reached 133 similar to 136g/L after 36h, indicating that the conversion rate was as high as 88%similar to 91%, and the productivity was 3.75g/L(.)h. There was almost a 70% increase in lactic acid production compared with the original strain RE3303.
引用
收藏
页码:205 / 216
页数:12
相关论文
共 50 条
  • [21] L-LACTIC ACID FERMENTATION BY RHIZOPUS ORYZAE USING DAIRY MANURE AS A NITROGEN SOURCE
    Yao, W.
    Wu, X.
    Zhu, J.
    Sun, B.
    Miller, C.
    TRANSACTIONS OF THE ASABE, 2009, 52 (06) : 2047 - 2054
  • [22] L-Lactic acid fermentation by culture of Rhizopus oryzae using ammonia as neutralizing agent
    Wang, Chung-Yih
    Lin, Chi-Tsai
    Sheu, Dey-Chyi
    Liu, Chih-Yu
    JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS, 2014, 45 (01) : 1 - 5
  • [23] Impacts of lignocellulose-derived inhibitors on L-lactic acid fermentation by Rhizopus oryzae
    Zhang, Li
    Li, Xin
    Yong, Qiang
    Yang, Shang-Tian
    Ouyang, Jia
    Yu, Shiyuan
    BIORESOURCE TECHNOLOGY, 2016, 203 : 173 - 180
  • [24] Study on fermentation of L-lactic acid by rhizopus
    Chen, Bi'e
    Liu, Zutong
    Qinghua Daxue Xuebao/Journal of Tsinghua University, 1993, 33 (06): : 91 - 96
  • [25] Improved oxygen transfer and increased l-lactic acid production by morphology control of Rhizopus oryzae in a static bed bioreactor
    Chotisubha-anandha, Nawakorn
    Thitiprasert, Sitanan
    Tolieng, Vasana
    Thongchul, Nuttha
    BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2011, 34 (02) : 163 - 172
  • [26] Improved oxygen transfer and increased l-lactic acid production by morphology control of Rhizopus oryzae in a static bed bioreactor
    Nawakorn Chotisubha-anandha
    Sitanan Thitiprasert
    Vasana Tolieng
    Nuttha Thongchul
    Bioprocess and Biosystems Engineering, 2011, 34 : 163 - 172
  • [27] Simultaneous saccharification and fermentation of xylo-oligosaccharides manufacturing waste residue for L-lactic acid production by Rhizopus oryzae
    Zhang, Li
    Li, Xin
    Yong, Qiang
    Yang, Shang-Tian
    Ouyang, Jia
    Yu, Shiyuan
    BIOCHEMICAL ENGINEERING JOURNAL, 2015, 94 : 92 - 99
  • [28] A new method studying the kinetics of l-lactic acid production by pellets Rhizopus oryzae in semi-continuous fermentation
    Xuefeng Wu
    Shaotong Jiang
    Min Zhang
    Shuizhong Luo
    Xingjiang Li
    Lijun Pan
    Zhi Zheng
    Mo Liu
    Annals of Microbiology, 2015, 65 : 1473 - 1480
  • [29] A new method studying the kinetics of L-lactic acid production by pellets Rhizopus oryzae in semi-continuous fermentation
    Wu, Xuefeng
    Jiang, Shaotong
    Zhang, Min
    Luo, Shuizhong
    Li, Xingjiang
    Pan, Lijun
    Zheng, Zhi
    Liu, Mo
    ANNALS OF MICROBIOLOGY, 2015, 65 (03) : 1473 - 1480
  • [30] Effect of different carbon sources on L(+)-lactic acid production by Rhizopus oryzae
    Bulut, S
    Elibol, M
    Ozer, D
    BIOCHEMICAL ENGINEERING JOURNAL, 2004, 21 (01) : 33 - 37