Generation of a human embryonic stem cell line expressing tetrameric Zoanthus sp green fluorescent protein: NERCe002-A-1

被引：0
|
作者：
Duan, Xingxiang [1 ]
Xie, Menghan [1 ]
Peng, Yingying [1 ]
Hu, Liang [1 ,2 ,3 ]
Yu, Juan [2 ]
Zeng, Sicong [1 ,2 ]
Wang, Yang [1 ]
Lu, Guangxiu [2 ,3 ,4 ]
Lin, Ge [1 ,2 ,3 ,4 ]
Sun, Yi [1 ,2 ,3 ]
机构：
[1] Cent South Univ, Sch Basic Med Sci, Inst Reprod & Stem Cell Engn, Changsha, Hunan, Peoples R China
[2] Natl Engn & Res Ctr Human Stem Cell, Changsha, Hunan, Peoples R China
[3] Minist Hlth, Key Lab Stem Cells & Reprod Engn, Changsha, Hunan, Peoples R China
[4] Reprod & Genet Hosp CITIC Xiangya, Changsha, Hunan, Peoples R China
来源：
STEM CELL RESEARCH | 2018年 / 28卷
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.scr.2018.01.033
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The human embryonic stem cell (hESC) line NERCe002-A-1 was generated through lentiviral transduction of the original NERCe002-A-1 hESC line with Zoanthus sp. green fluorescent protein (ZsGreen). Cells that expressed ZsGreen showed a >8.6-fold increase in fluorescence intensity compared with that of cells that expressed enhanced green fluorescent protein. The fluorescent hESC line can aid in identification of biological characteristics in vitro and in vivo by tracking cell growth, migration, and differentiation. Characteristic tests confirmed that the NERCe002-A-1 cell line expressed typical markers of pluripotency and had the capability to form the three germ layers in vivo. (c) 2018 The Authors. Published by Elsevier B.V.
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收藏
页码：6 / 10
页数：5
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