An open tubular capillary electrochromatography column with porous inner surface for protein separation

被引:26
|
作者
Liu, Haiyan [1 ]
Li, Xiaohui [1 ]
Huang, Lanqi [1 ]
Zhang, Lingyi [1 ]
Zhang, Weibing [1 ]
机构
[1] E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China
基金
中国国家自然科学基金;
关键词
Proteins; Open tubular capillary; electrochromatography; Porogen; Porous inner surface; STATIONARY PHASES; POLYMER MONOLITHS; PEPTIDES; LAYER; MICROREACTOR; MORPHOLOGY; EFFICIENCY; POROGEN;
D O I
10.1016/j.ab.2013.06.024
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An open tubular capillary electrochromatography (OTCEC) column using sole porogen to form porous inner surface has been developed. The porous layer was coated on the capillary inner wall by in situ polymerization in the presence of porogen. The results show that the columns using 1-propanol as sole porogen are appropriate for protein separation. It has higher separation efficiency than the column with the usual coporogen due to much more micropores and mesopores on the porous surface and a higher specific surface area. In addition, the sensitivity of the prepared OTCEC column was improved greatly compared with the dynamically coated capillary with polyvinylpyrrolidone. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:186 / 188
页数:3
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