Dual-micropillar-based microfluidic platform for single embryonic stem cell-derived neuronal differentiation

被引：9

作者：

Lee, Jong Min ^{[1
]}

Kim, Ji-eun ^{[1
]}

Borana, Jayant ^{[2
]}

Chung, Bong Hyun ^{[3
]}

Chung, Bong Geun ^{[4
]}

机构：

[1] Hanyang Univ, Dept Bionano Technol, Ansan, South Korea

[2] Indian Inst Technol, Dept Chem Engn, Gauhati, India

[3] Korea Res Inst Biosci & Biotechnol, BioNanotechnol Res Ctr, Taejon, South Korea

[4] Sogang Univ, Dept Mech Engn, Seoul, South Korea

来源：

ELECTROPHORESIS | 2013年 / 34卷 / 13期

基金：

新加坡国家研究基金会;

关键词：

Embryonic stem cell; Microfluidic device; Neuronal differentiation; CULTURE; ARRAY;

D O I：

10.1002/elps.201200578

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed the dual-micropillar-based microfluidic platform to direct embryonic stem (ES) cell fate. 4 x 4 dual-micropillar-based microfluidic platform consisted of 16 circular-shaped outer micropillars and 8 saddle-shaped inner micropillars in which single ES cells were cultured. We hypothesized that dual-micropillar arrays would play an important role in controlling the shear stress and cell docking. Circular-shaped outer micropillars minimized the shear stress, whereas saddle-shaped inner micropillars allowed for docking of individual ES cells. We observed the effect of saddle-shaped inner micropillars on cell docking in response to hydrodynamic resistance. We also demonstrated that ES cells cultured for 6 days within the dual-micropillar-based microfluidic platform differentiated into neural-like cells. Therefore, this dual-micropillar-based microfluidic platform could be a potentially powerful method for screening of lineage commitments of single ES cells.

引用

页码：1931 / 1938

页数：8

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