EFFECT OF ANTI-TNF-α MAB ON NF-κB NUCLEAR TRANSLOCATION AND PROLIFERATION OF HUMAN HEPG2 CELLS

被引：0

作者：

Zhang Haijian ^{[1
]}

Yao Dengfu ^{[1
]}

Wu Wei ^{[1
]}

Qiu Liwei ^{[1
]}

Qin Chenglin ^{[1
]}

Yang Junling ^{[1
]}

机构：

[1] Nantong Univ, Affiliated Hosp, Res Ctr Clin Med, Nantong 226001, Peoples R China

来源：

PROGRESS ON POST-GENOME TECHNOLOGIES AND MODERN NATURAL PRODUCTS, 2011 | 2011年

关键词：

TNF-alpha mAb; NF-kappa B; cell cycle; apoptosis; HEPATOCELLULAR-CARCINOMA; MOLECULAR TARGET; CANCER; THERAPY; PROGRESSION;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The activation of NF-kappa B induced by tumor necrosis factor-alpha (TNF-alpha) is closely related to hepatocarcinogenesis and drug tolerance, but the influence of inhibiting NF-kappa B Activation by TNF-alpha mAb in hepatoma cells is still poorly understood up to now. In this paper, we investigated the effect of TNF-alpha mAb on NF-kappa B activation and nuclear translocation by many methods, such as ELISA, Western Blot, qPCR, immunohistochemistry. The results indicated that the expression level of NF-kappa B mRNA was significantly inhibited. Besides, NF-kappa B activation and nuclear translocation were significantly inhibited by TNF-alpha mAb in HepG2 cells. So it can be seen that intervention with TNF-alpha mAb is expected to become a new method of HCC targeted therapy.

引用

页码：171 / 173

页数：3

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