Selective enrichment of phosphopeptides by titania nanoparticles coated magnetic carbon nanotubes

被引:31
|
作者
Yan, Yinghua [1 ,2 ]
Zheng, Zhifang [1 ,2 ]
Deng, Chunhui [1 ,2 ]
Zhang, Xiangmin [1 ,2 ]
Yang, Pengyuan [1 ,2 ]
机构
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[2] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
基金
高等学校博士学科点专项科研基金; 中国国家自然科学基金;
关键词
MagCNTs@TiO2 composites; Selective enrichment; Phosphopeptides; Mass spectrometry analysis; SOLID-PHASE EXTRACTION; TIO2 NANOCRYSTAL CLUSTERS; MASS-SPECTROMETRY; FACILE SYNTHESIS; PEPTIDES; NANOCOMPOSITES; MICROSPHERES; SAMPLES;
D O I
10.1016/j.talanta.2013.09.036
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Selective enrichment of phosphoproteins or phosphopeptides from complex mixtures is essential for mass spectrometry (MS)-based phosphoproteomics. In this work, for the first time, titania nanoparticles coated magnetic carbon nanotubes (denoted as MagCNTs@TiO2 composites) were synthesized through a facile but effective solvothermal reaction for selective enrichment of phosphopeptides. The MagCNTs@TiO2 material demonstrated low limit of detection (20 fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein BSA and a phosphorylated protein beta-casein with molar ratios of BSA/beta-casein up to 200:1. In addition, the high magnetic susceptibility allowed convenient separation of the target peptides by magnetic separation. Experimental results demonstrated that the MagCNTs@TiO2 composites showed excellent potential for the selective enrichment of phosphopeptides for MS analysis. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:14 / 20
页数:7
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