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Selective enrichment of phosphopeptides by titania nanoparticles coated magnetic carbon nanotubes
被引:31
|作者:
Yan, Yinghua
[1
,2
]
Zheng, Zhifang
[1
,2
]
Deng, Chunhui
[1
,2
]
Zhang, Xiangmin
[1
,2
]
Yang, Pengyuan
[1
,2
]
机构:
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[2] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
来源:
基金:
高等学校博士学科点专项科研基金;
中国国家自然科学基金;
关键词:
MagCNTs@TiO2 composites;
Selective enrichment;
Phosphopeptides;
Mass spectrometry analysis;
SOLID-PHASE EXTRACTION;
TIO2 NANOCRYSTAL CLUSTERS;
MASS-SPECTROMETRY;
FACILE SYNTHESIS;
PEPTIDES;
NANOCOMPOSITES;
MICROSPHERES;
SAMPLES;
D O I:
10.1016/j.talanta.2013.09.036
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
Selective enrichment of phosphoproteins or phosphopeptides from complex mixtures is essential for mass spectrometry (MS)-based phosphoproteomics. In this work, for the first time, titania nanoparticles coated magnetic carbon nanotubes (denoted as MagCNTs@TiO2 composites) were synthesized through a facile but effective solvothermal reaction for selective enrichment of phosphopeptides. The MagCNTs@TiO2 material demonstrated low limit of detection (20 fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein BSA and a phosphorylated protein beta-casein with molar ratios of BSA/beta-casein up to 200:1. In addition, the high magnetic susceptibility allowed convenient separation of the target peptides by magnetic separation. Experimental results demonstrated that the MagCNTs@TiO2 composites showed excellent potential for the selective enrichment of phosphopeptides for MS analysis. (C) 2013 Elsevier B.V. All rights reserved.
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页码:14 / 20
页数:7
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