Essential Oil from Eucalyptus benthamii Maiden et Cambage Reduces Nitric Oxide Production in Lipopolysaccharide-induced Murine Peritoneal Macrophages

被引：0
|
作者：
Doell-Boscardin, Patricia M. [3 ]
Almeida, Martinha A. [1 ]
Nakashima, Tomoe [3 ]
Padila De Paula, Josiane de F. [1 ]
Kanunfre, Carla C. [2 ]
Farago, Paulo V. [1 ]
机构：
[1] Univ Estadual Ponta Grossa, Postgrad Program Pharmaceut Sci, BR-84030900 Ponta Grossa, Brazil
[2] Univ Estadual Ponta Grossa, Dept Gen Biol, BR-84030900 Ponta Grossa, Brazil
[3] Univ Fed Parana, Postgrad Program Pharmaceut Sci, BR-80210170 Curitiba, Parana, Brazil
来源：
LATIN AMERICAN JOURNAL OF PHARMACY | 2012年 / 31卷 / 04期
关键词：
alpha-pinene; Essential oil; Eucalyptus benthamii; Myrtaceae; Nitric oxide production; GROWTH;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Few studies are concerned about the essential oil extracted from leaves of Eucalyptus benthamii Maiden et Cambage that shows high content of alpha-pinene. The goal of this paper was to investigate the in vitro effect of the essential oil of E. benthamii and alpha-pinene on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse peritoneal macrophages. Macrophages were harvested by washing with phosphate buffered saline and cultured with 10 mu g/mL LPS. Three concentrations (5, 10, and 20 mu g/mL) of the essential oil of E. benthamii and alpha-pinene were investigated. Nitrite levels were measured based on the Griess reaction, an indirect assay for NO production. The essential oil of E. benthamii significantly suppressed NO production in murine peritoneal macrophages at 10 and 20 mu g/mL. In contrast, alpha-pinene did not inhibit NO production.
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收藏
页码：545 / 548
页数：4
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