Nanoscale membrane activity of surfactins: Influence of geometry, charge and hydrophobicity

被引:39
|
作者
Francius, Gregory [3 ]
Dufour, Samuel [1 ]
Deleu, Magali [1 ]
Papot, Michel [1 ]
Mingeot-Leclercq, Marie-Paule [2 ]
Dufrene, Yves F. [3 ]
机构
[1] Fac Univ Sci Agron Gembloux, Unite Chim Ind, B-5030 Gembloux, Belgium
[2] Univ Catholique Louvain, Unite Pharmacol Cellulaire & Mol, B-1200 Brussels, Belgium
[3] Univ Catholique Louvain, Unite Chim Interfaces, B-1348 Louvain, Belgium
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2008年 / 1778卷 / 10期
关键词
AFM; Analogs; Micelles; Supported lipid membranes; Surfactin;
D O I
10.1016/j.bbamem.2008.03.023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We used real-time atomic force microscopy (AFM) to visualize the interactions between supported lipid membranes and well-defined surfactin analogs, with the aim to understand the influence of geometry, charge and hydrophobicity. AFM images of mixed dioleoylphosphatidylcholine/dipalmitoylphosphaticlylcholine (DOPC/DPPC) bilayers recorded after injection of cyclic surfactin at 1 mM, i.e. well-above the critical micelle concentration, revealed a complete solubilization of the bilayers within 30 min. A linear analog having the same charge and acyl chains was able to solubilize DOPC, but not DPPC, and to promote redeposition leading eventually to a new bilayer. Increasing the charge of the polar head or the length of the acyl chains of the analogs lead to the complete solubilization of both DOPC and DPPC, thus to a stronger membrane activity. Lastly. we found that at low surfactin concentrations (40 mu M). DPPC domains were always resistant to solubilization. These data demonstrate the crucial role played by geometry, charge and hydrophobicity in modulating the membrane activity (solubilization, redeposition) of surfactin. Also, this study suggests that synthetic analogs are excellent candidates for developing new surfactants with tunable, well-defined properties for medical and biotechnological applications. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:2058 / 2068
页数:11
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