Efficient and quantitative high-throughput tRNA sequencing

被引：0

作者：

Zheng, Guanqun ^{[1
]}

Qin, Yidan ^{[2
]}

Clark, Wesley C. ^{[1
]}

Dai, Qing ^{[3
]}

Yi, Chengqi ^{[1
]}

He, Chuan ^{[1
,3
,4
,5
]}

Lambowitz, Alan M. ^{[2
]}

Pan, Tao ^{[1
]}

机构：

[1] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA

[2] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA

[3] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

[4] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA

[5] Univ Chicago, Howard Hughes Med Inst, Chicago, IL 60637 USA

来源：

NATURE METHODS | 2015年 / 12卷 / 09期

基金：

美国国家卫生研究院;

关键词：

ESCHERICHIA-COLI; DNA-DAMAGE; OXIDATIVE DEMETHYLATION; ALKB; REPAIR; GENES; SEQ;

D O I：

10.1038/NMETH.3478

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered demethylases to remove base methylations and a highly processive thermostable group II intron reverse transcriptase to overcome these obstacles. Our method, DM-tRNA-seq, should be applicable to investigations of tRNA in all organisms.

引用

页码：835 / +

页数：5

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