The use of concentrated extenders to improve the efficacy of cryopreservation in whitefish spermatozoa

被引：10
|
作者：
Ciereszko, A. [1 ]
Dietrich, G. J. [1 ]
Nynca, J. [1 ]
Liszewska, E. [1 ]
Karol, H. [1 ]
Dobosz, S. [2 ]
机构：
[1] Polish Acad Sci, Inst Anim Reprod & Food Res, Dept Gamete & Embryo Biol, PL-10748 Olsztyn, Poland
[2] Inland Fisheries Inst, Dept Salmonid Fish Res, PL-83300 Rutki, Zukowo, Poland
来源：
AQUACULTURE | 2013年 / 408卷
关键词：
Fish; Milt; Freezing; Sperm motility; LAVARETUS L. SEMEN; RAINBOW-TROUT; COREGONUS-LAVARETUS; SPERM CONCENTRATION;
D O I：
10.1016/j.aquaculture.2013.05.016
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The objective of this work was to test the effects of three dilutions of semen to glucose-methanol extender ratios 1:3, 1:1 and 3:1 on the sperm motility characteristics of diluted and cryopreserved whitefish spermatozoa. The final concentration of extender compounds was achieved using concentrated extenders. The application of 3:1 dilution ratio allowed for the cryopreservation of up to 3 times more spermatozoa than using the currently recommended method (dilution 1:3). However, changes in sperm motility parameters were observed in response to a lower dilution ratio of semen. Further studies should be carried out to optimize this method regarding particular steps for cryopreservation technologies, especially concerning dilution. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：30 / 33
页数：4
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