Multi-spectral endogenous fluorescence imaging for bacterial differentiation

被引：0

作者：

Chernomyrdin, Nikita V. ^{[1
]}

Babayants, Margarita V.

Korotkov, Oleg V. ^{[2
]}

Kudrin, Konstantin G. ^{[3
]}

Rimskaya, Elena N. ^{[3
]}

Shikunova, Irina A. ^{[4
]}

Kurlov, Vladimir N. ^{[4
]}

Cherkasova, Olga P. ^{[5
]}

Komandin, Gennady A. ^{[6
]}

Reshetov, Igor V. ^{[3
]}

Zaytsev, Kirill I. ^{[1
,3
,6
]}

机构：

[1] Bauman Moscow State Tech Univ, Moscow 105005, Russia

[2] State Humanitarian Technol Univ, Orekhovo Zyevo 142600, Russia

[3] Sechenov First Moscow State Med Univ, Moscow 119991, Russia

[4] RAS, Inst Solid State Phys, Chernogolovka 142432, Russia

[5] RAS, Inst Laser Phys, Siberian Branch, Novosibirsk 630090, Russia

[6] RAS, Prokhorov Gen Phys Inst, Moscow 119991, Russia

来源：

CLINICAL AND PRECLINICAL OPTICAL DIAGNOSTICS | 2017年 / 10411卷

基金：

俄罗斯科学基金会;

关键词：

endogenous fluorescence; multi-spectral fluorescence imaging; bacterial differentiation; BASAL-CELL CARCINOMA; IN-VIVO APPLICATIONS; CORRELATION SPECTROSCOPY; BARRETTS-ESOPHAGUS; COMPONENT ANALYSIS; SKIN; MICROSCOPY; DIAGNOSIS; REFLECTANCE; CANCERS;

D O I：

10.1117/12.2285065

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper, the multi-spectral endogenous fluorescence imaging was implemented for bacterial differentiation. The fluorescence imaging was performed using a digital camera equipped with a set of visual bandpass filters. Narrowband 365 nm ultraviolet radiation passed through a beam homogenizer was used to excite the sample fluorescence. In order to increase a signal-to-noise ratio and suppress a non-fluorescence background in images, the intensity of the UV excitation was modulated using a mechanical chopper. The principal components were introduced for differentiating the samples of bacteria based on the multi-spectral endogenous fluorescence images.

引用

页数：7

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