We developed a novel single-step virus detection system using the fluorescent molecule with a hairpin primer on the reverse transcription (RT)-polymerase chain reaction (PCR). Primers that are specific to Hepatitis C virus (HCV) having a hairpin tag at the 5 '-end were used for RT and PCR. The HCV-RNA template was reverse transcribed by reverse transcriptase with the hairpin primer (HP), and the resulting cDNA was amplified directly by HP-PCR (RT-HP-PCR). Using the RT-HP-PCR, we succeeded in demonstrating the detection of HCV-RNA in single-step. The RT-HP-PCR selectively detected HCV-RNA extracted from patients' sera containing contaminant materials. The method could be applicable to the quantitative detection of HCV. (c) 2013 Elsevier Ltd. All rights reserved.
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Natl Inst Virol, Maximum Containment Lab, Sus Rd, Pune 410021, Maharashtra, IndiaNatl Inst Virol, Maximum Containment Lab, Sus Rd, Pune 410021, Maharashtra, India
Chaubal, Gouri
Sarkale, Prasad
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Natl Inst Virol, Maximum Containment Lab, Sus Rd, Pune 410021, Maharashtra, IndiaNatl Inst Virol, Maximum Containment Lab, Sus Rd, Pune 410021, Maharashtra, India
Sarkale, Prasad
Kore, Pravin
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Natl Inst Virol, Maximum Containment Lab, Sus Rd, Pune 410021, Maharashtra, IndiaNatl Inst Virol, Maximum Containment Lab, Sus Rd, Pune 410021, Maharashtra, India
Kore, Pravin
Yadav, Pragya
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Natl Inst Virol, Maximum Containment Lab, Sus Rd, Pune 410021, Maharashtra, IndiaNatl Inst Virol, Maximum Containment Lab, Sus Rd, Pune 410021, Maharashtra, India
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Madras Vet Coll, Dept Vet Pharmacol & Toxicol, Chennai 600007, Tamil Nadu, IndiaTamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Chennai 600051, Tamil Nadu, India
Srinivasan, M. R.
Vijay, K.
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Tamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Chennai 600051, Tamil Nadu, IndiaTamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Chennai 600051, Tamil Nadu, India
Vijay, K.
Karuppannan, A. K.
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Tamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Chennai 600051, Tamil Nadu, IndiaTamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Chennai 600051, Tamil Nadu, India
Karuppannan, A. K.
Ramesh, S.
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Madras Vet Coll, Dept Vet Pharmacol & Toxicol, Chennai 600007, Tamil Nadu, IndiaTamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Chennai 600051, Tamil Nadu, India
Ramesh, S.
Reddy, Y. Krishnamohan
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Tamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Chennai 600051, Tamil Nadu, IndiaTamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Chennai 600051, Tamil Nadu, India