Flow cytometry assay for the detection of single-copy DNA in human lymphocytes

被引：2
|
作者：
Uno, Naoki [1 ]
Kaku, Norihito [1 ]
Morinaga, Yoshitomo [1 ]
Hasegawa, Hiroo [1 ]
Yanagihara, Katsunori [1 ]
机构：
[1] Nagasaki Univ, Dept Lab Med, Grad Sch Biomed Sci, Nagasaki 8528501, Japan
来源：
NUCLEIC ACIDS RESEARCH | 2020年 / 48卷 / 15期
关键词：
IN-SITU HYBRIDIZATION; MESSENGER-RNA; NUCLEOTIDE POLYMORPHISMS; CELLS; PCR; AMPLIFICATION; PROVIRUS;
D O I：
10.1093/nar/gkaa515
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization, flow cytometric detection of specific genomic DNA sequences has not been established. Here, we present a flow cytometry assay for detection of single-copy genomic sequences in human lymphocytes using in situ PCR with universal energy transfer-labelled primers.
引用
收藏
页数：7
相关论文
共 50 条
  • [41] Random DNA fragmentation allows detection of single-copy, single-exon alterations of copy number by oligonucleotide array CGH in clinical FFPE samples
    Hostetter, Galen
    Kim, Su Young
    Savage, Stephanie
    Gooden, Gerald C.
    Barrett, Michael
    Zhang, Jian
    Alla, Lalitamba
    Watanabe, April
    Einspahr, Janine
    Prasad, Anil
    Nickoloff, Brian J.
    Carpten, John
    Trent, Jeffrey
    Alberts, David
    Bittner, Michael
    NUCLEIC ACIDS RESEARCH, 2010, 38 (02) : e9.1 - e9.12
  • [42] Single-copy detection of somatic variants from solid and liquid biopsy
    Ana-Luisa Silva
    Paulina Klaudyna Powalowska
    Magdalena Stolarek
    Eleanor Ruth Gray
    Rebecca Natalie Palmer
    Bram Herman
    Cameron Alexander Frayling
    Barnaby William Balmforth
    Scientific Reports, 11
  • [43] DETECTION OF MORE THAN 500 SINGLE-COPY RFLPS BY RANDOM SCREENING
    SCHUMM, J
    KNOWLTON, R
    BRAMAN, J
    BARKER, D
    VOVIS, G
    AKOTS, G
    BROWN, V
    GRAVIUS, T
    HELMS, C
    HSIAO, K
    REDIKER, K
    THURSTON, J
    BOTSTEIN, D
    DONISKELLER, H
    CYTOGENETICS AND CELL GENETICS, 1985, 40 (1-4): : 739 - 739
  • [44] Single-copy detection of somatic variants from solid and liquid biopsy
    Silva, Ana-Luisa
    Powalowska, Paulina Klaudyna
    Stolarek, Magdalena
    Gray, Eleanor Ruth
    Palmer, Rebecca Natalie
    Herman, Bram
    Frayling, Cameron Alexander
    Balmforth, Barnaby William
    SCIENTIFIC REPORTS, 2021, 11 (01)
  • [45] Detection of nascent RNA, single-copy DNA and protein localization by immunoFISH in mouse germ cells and preimplantation embryos
    Satoshi H Namekawa
    Jeannie T Lee
    Nature Protocols, 2011, 6 : 270 - 284
  • [46] Detection of nascent RNA, single-copy DNA and protein localization by immunoFISH in mouse germ cells and preimplantation embryos
    Namekawa, Satoshi H.
    Lee, Jeannie T.
    NATURE PROTOCOLS, 2011, 6 (03) : 270 - 284
  • [47] SINGLE-COPY DNA RELATIONSHIPS BETWEEN DIPLOID AND TETRAPLOID TELEOSTEAN FISH SPECIES
    SCHMIDTKE, J
    KANDT, I
    CHROMOSOMA, 1981, 83 (02) : 191 - 197
  • [48] DNA METHYLATION ANALYSES OF SINGLE-COPY LOCI IN 2 PATIENTS WITH ICF SYNDROME
    HORTNAGEL, K
    SCHUFFENHAUER, S
    PETROPOULOU, T
    BUCHHOLZ, B
    KRAFT, S
    HINKEL, GK
    BELOHRADSKY, B
    MEITINGER, T
    AMERICAN JOURNAL OF HUMAN GENETICS, 1995, 57 (04) : 757 - 757
  • [49] A novel set of single-copy nuclear DNA markers for the genetic study of Salicaceae
    Du, S. H.
    Wang, Z. S.
    Zhang, J. G.
    GENETICS AND MOLECULAR RESEARCH, 2014, 13 (03): : 4911 - 4917
  • [50] RAPID ASSESSMENT OF SINGLE-COPY NUCLEAR-DNA VARIATION IN DIVERSE SPECIES
    SLADE, RW
    MORITZ, C
    HEIDEMAN, A
    HALE, PT
    MOLECULAR ECOLOGY, 1993, 2 (06) : 359 - 373
12345