Europium(III)-sensitized chemiluminescence determination of ibuprofen in pharmaceutical preparations and biological fluids

Chemiluminescence reaction of the system containing europium(III) ion, KMnO4, Na2SO3, and ibuprofen was investigated for the determination of ibuprofen. The introduction of Eu(III) ion into the system of KMnO4-Na2SO3-ibuprofen caused a significant increase in the chemiluminescence signal. The increment of the chemiluminescence signal is proportional to ibuprofen concentration in the range of 5.0 x 10(-8) - 5.0 x 10(-6) g/ml with a detection limit of 1 x 10(-8) g/ml. The relative standard deviation for 1.0 x 10(-7) g/ml ibuprofen solution was 1.7% (n = 11). The proposed method was successfully applied to determine ibuprofen in tablets and human plasma.