Stress-induced lncRNAs evade nuclear degradation and enter the translational machinery

被引:28
|
作者
Galipon, Josephine [1 ]
Miki, Atsuko [1 ]
Oda, Arisa [1 ]
Inada, Toshifumi [2 ]
Ohta, Kunihiro [1 ,3 ]
机构
[1] Univ Tokyo, Grad Sch Sci, Dept Biophys & Biochem, Bunkyo Ku, Tokyo 1130033, Japan
[2] Tohoku Univ, Grad Sch Pharmaceut Sci, Dept Mol Biopharm & Genet, Aoba Ku, Sendai, Miyagi 9808578, Japan
[3] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan
基金
日本学术振兴会;
关键词
MESSENGER-RNA DECAY; LONG NONCODING RNAS; OPEN READING FRAMES; FISSION YEAST; BINDING PROTEINS; GENE-EXPRESSION; TRANSCRIPTS; UPSTREAM; TERMINATION; PROMOTERS;
D O I
10.1111/gtc.12042
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Long noncoding RNAs (lncRNAs) play important roles in the regulation of gene expression. In fission yeast, glucose starvation triggers a transcriptional cascade of polyadenylated lncRNAs in the upstream region of the fructose-1,6-bisphosphatase gene (fbp1+), which is correlated with stepwise chromatin remodeling and necessary for the massive induction of fbp1+ mRNA. Here, we show that these novel metabolic stress-induced lncRNAs (mlonRNAs) are 5-capped, less stable than fbp1+ mRNA and sensitive to a certain extent to the nuclear exosome cofactor Rrp6. However, most mlonRNAs seem to escape nuclear degradation and are exported to the cytoplasm, where they localize to polysomes precisely during glucose starvation-induced global translation inhibition. It is likely that ribosomes tend to accumulate in the upstream region of mlonRNAs. Although mlonRNAs contain an unusual amount of upstream AUGs (uAUGs) and small open reading frames (uORFs), they escape Upf1-mediated targeting to the non-sense-mediated decay (NMD) pathway. The deletion of Upf1 had no effect on mlonRNA stability, but considerably destabilized fbp1+ mRNA, hinting toward a possible novel role of Upf1. Our findings suggest that the stability of mlonRNAs is distinctly regulated from mRNA and previously described noncoding transcripts.
引用
收藏
页码:353 / 368
页数:16
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