CCN1 Promotes VEGF Production in Osteoblasts and Induces Endothelial Progenitor Cell Angiogenesis by Inhibiting miR-126 Expression in Rheumatoid Arthritis

被引:74
|
作者
Chen, Cheng-Yu [1 ]
Su, Chen-Ming [2 ]
Hsu, Chin-Jung [3 ,4 ]
Huang, Chien-Chung [5 ,6 ]
Wang, Shih-Wei [7 ]
Liu, Shih-Chia [8 ]
Chen, Wei-Cheng [8 ]
Fuh, Lih-Jyh [9 ]
Tang, Chih-Hsin [1 ,10 ,11 ]
机构
[1] China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan
[2] Wenzhou Med Univ, Dept Biomed Sci Lab, Affiliated Dongyang Hosp, Dongyang, Peoples R China
[3] China Med Univ, Sch Chinese Med, Taichung, Taiwan
[4] China Med Univ Hosp, Dept Orthoped Surg, Taichung, Taiwan
[5] China Med Univ Hosp, Dept Internal Med, Div Immunol & Rheumatol, Taichung, Taiwan
[6] China Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan
[7] Mackay Med Coll, Dept Med, New Taipei, Taiwan
[8] Mackay Mem Hosp, Dept Orthopaed, Taipei, Taiwan
[9] China Med Univ Hosp, Dept Prosthodont, Taichung, Taiwan
[10] China Med Univ, Sch Med, Dept Pharmacol, Taichung, Taiwan
[11] Asia Univ, Coll Hlth Sci, Dept Biotechnol, Taichung, Taiwan
关键词
CCN1; VEGF; OSTEOBLASTS; ARTHRITIS; FACTOR-DEPENDENT ANGIOGENESIS; FIBROBLAST-LIKE SYNOVIOCYTES; HUMAN SYNOVIAL FIBROBLASTS; GROWTH-FACTOR; IN-VITRO; INFLAMMATION; CYR61; PROLIFERATION; ALPHA; SRC;
D O I
10.1002/jbmr.2926
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Angiogenesis is the formation of new capillaries from preexisting vasculature. The perpetuation of angiogenesis plays a critical role in the pathogenesis of various disease states including rheumatoid arthritis (RA). Cysteine-rich 61 (Cyr61 or CCN1) is an important proinflammatory cytokine in RA. Here, we investigated the role of CCN1 in angiogenesis associated with vascular endothelial growth factor (VEGF) production and osteoblasts. We found higher expression of CCN1 and VEGF in synovial fluid from RA patients compared with healthy controls. CCN1 induced VEGF expression in osteoblasts and increased endothelial progenitor cells (EPCs) angiogenesis by inhibiting miR-126 via the protein kinase C-alpha (PKC-) signaling pathway. CCN1 knockdown inhibited angiogenesis in both in vitro and in vivo models. Inhibition of CCN1 expression with lentiviral vectors expressing short hairpin RNA (shRNA) ameliorated articular swelling, cartilage erosion, and angiogenesis in the ankle joint of mice with collagen-induced arthritis (CIA). Our study is the first to describe how CCN1 promotes VEGF expression in osteoblasts and increased EPCs angiogenesis in RA disease. CCN1 may serve as a potential target for RA treatment. (c) 2016 American Society for Bone and Mineral Research.
引用
收藏
页码:34 / 45
页数:12
相关论文
共 37 条
  • [21] MiR-126 enhances VEGF expression in induced pluripotent stem cell-derived retinal neural stem cells by targeting spred-1
    Ye, Lin
    Peng, Yun
    Mo, Jinsong
    Yao, Yuanyuan
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2018, 11 (02): : 1023 - 1030
  • [22] circ_0002970 promotes fibroblast-like synoviocytes invasion and the inflammatory response through Hippo/YAP signaling to induce CTGF/CCN1 expression in rheumatoid arthritis
    Haoran Kong
    Yushuang Qiao
    Dahu Qi
    Shixin Zhao
    Zhiming Cao
    Junhao Feng
    Yitong Li
    Yunke Liu
    Tao Liu
    Arthritis Research & Therapy, 27 (1)
  • [23] Annexin-1-mediated Endothelial Cell Migration and Angiogenesis Are Regulated by Vascular Endothelial Growth Factor (VEGF)-induced Inhibition of miR-196a Expression
    Pin, Anne-Laure
    Houle, Francois
    Fournier, Patrick
    Guillonneau, Maeva
    Paquet, Eric R.
    Simard, Martin J.
    Royal, Isabelle
    Huot, Jacques
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (36) : 30541 - 30551
  • [24] Stromal cell-derived factor-1α gene transfer induces therapeutic angiogenesis by recruiting endothelial progenitor cells via the VEGF/eNOS pathway
    Hiasa, K
    Inoue, S
    Ishibashi, M
    Zhao, C
    Otani, K
    Kitamoto, S
    Usui, M
    Egashira, K
    Takeshita, A
    CIRCULATION, 2002, 106 (19) : 62 - 63
  • [25] Interleukin-18 induces production of vascular endothelial growth factor (VEGF) in rheumatoid arthritis synovial fibroblast through AP-1 activation
    Cho, ML
    Kim, KW
    Min, SY
    Yoon, CH
    Lee, SH
    Park, SH
    Kim, HY
    FASEB JOURNAL, 2005, 19 (04): : A327 - A327
  • [26] Interleukin-18 induces production of vascular endothelial growth factor (VEGF) in rheumatoid arthritis synovial fibroblast through AP-1 activation
    Yoon, CH
    Jung, YK
    Cho, ML
    Min, SY
    Hong, JH
    Kang, HJ
    Park, KS
    Lee, SH
    Park, SH
    Cho, CS
    Jue, DM
    Kim, HY
    ARTHRITIS AND RHEUMATISM, 2004, 50 (09): : S152 - S153
  • [27] Interleukin-18 induces vascular endothelial growth factor (VEGF) production in rheumatoid arthritis synovial fibroblast through AP-1 activation
    Kim, H
    Cho, M
    Jung, Y
    Min, S
    Lee, S
    Park, S
    Cho, C
    ANNALS OF THE RHEUMATIC DISEASES, 2004, 63 : 135 - 135
  • [28] Interleukin-18 induces the production of vascular endothelial growth factor (VEGF) in rheumatoid arthritis synovial fibroblasts via AP-1-dependent pathways
    Cho, ML
    Jung, YO
    Moon, YM
    Min, SY
    Yoon, CH
    Leea, SH
    Park, SH
    Cho, CS
    Jue, DM
    Kim, HY
    IMMUNOLOGY LETTERS, 2006, 103 (02) : 159 - 166
  • [29] MiR-205 promotes endothelial progenitor cell angiogenesis and deep vein thrombosis recanalization and resolution by targeting PTEN to regulate Akt/autophagy pathway and MMP2 expression
    Sun, Li-Li
    Xiao, Lun
    Du, Xiao-Long
    Hong, Lei
    Li, Cheng-Long
    Jiao, Jian
    Li, Wen-Dong
    Li, Xiao-Qiang
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2019, 23 (12) : 8493 - 8504
  • [30] Downregulation of miR-125a-5p Promotes Endothelial Progenitor Cell Migration and Angiogenesis and Alleviates Deep Vein Thrombosis in Mice Via Upregulation of MCL-1
    Jingfan Yu
    Yiqi Jin
    Chen Xu
    Changwen Fang
    Zhixuan Zhang
    Lei Chen
    Guoxiong Xu
    Molecular Biotechnology, 2023, 65 : 1664 - 1678