Amperometric sulfide detection using Coprinus cinereus peroxidase immobilized on screen printed electrode in an enzyme inhibition based biosensor

被引:60
|
作者
Savizi, Iman Shahidi Pour [1 ]
Kariminia, Hamid-Reza [1 ]
Ghadiri, Mohammad [1 ]
Roosta-Azad, Reza [1 ]
机构
[1] Sharif Univ Technol, Dept Chem & Petr Engn, Tehran, Iran
来源
BIOSENSORS & BIOELECTRONICS | 2012年 / 35卷 / 01期
关键词
Sulfide detection; Screen printed electrode; Enzyme inhibition biosensor; Coprinus cinereus peroxidase; HORSERADISH-PEROXIDASE; SPECTROPHOTOMETRIC DETERMINATION; HYDROGEN-SULFIDE; MICROELECTRODES; NANOPARTICLES; WATERS; SENSOR; IONS;
D O I
10.1016/j.bios.2012.03.004
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In the present work, an amperometric inhibition biosensor for the determination of sulfide has been fabricated by immobilizing Coprinus cinereus peroxidase (CIP) on the surface of screen printed electrode (SPE). Chitosan/acrylamide was applied for immobilization of peroxidase on the working electrode. The amperometric measurement was performed at an applied potential of -150 mV versus Ag/AgCl with a scan rate of 100 mV in the presence of hydroquinone as electron mediator and 0.1 M phosphate buffer solution of pH 6.5. The variables influencing the performance of sensor including the amount of substrate, mediator concentration and electrolyte pH were optimized. The determination of sulfide can be achieved in a linear range of 1.09-16.3 mu M with a detection limit of 0.3 mu M. Developed sensor showed quicker response to sulfide compared to the previous developed sulfide biosensors. Common anions and cations in environmental water did not interfere with sulfide detection by the developed biosensor. Cyanide interference on the enzyme inhibition caused 43.25% error in the calibration assay which is less than the amounts reported by previous studies. Because of high sensitivity and the low-cost of SPE, this inhibition biosensor can be successfully used for analysis of environmental water samples. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:297 / 301
页数:5
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