Calcium Phosphate Transfection of Primary Hippocampal Neurons

被引：21
|
作者：
Sun, Miao [1 ]
Bernard, Laura P. [1 ]
DiBona, Victoria L. [1 ]
Wu, Qian [1 ]
Zhang, Huaye [1 ]
机构：
[1] Rutgers State Univ, Robert Wood Johnson Med Sch, Dept Neurosci & Cell Biol, Piscataway, NJ 08855 USA
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2013年 / 81期
关键词：
Neuroscience; Issue; 81; Primary hippocampal neuron; calcium phosphate transfection; Coculture; astroglial cells; DNA; EFFICIENCY;
D O I：
10.3791/50808
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.
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页数：7
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