Properties of the Bacillus pumilus Glutamyl endopeptidase at different growth stages of its recombinant strain

被引：1
|
作者：
Balaban, N. P. [1 ]
Danilova, Yu V. [1 ]
Shamsutdinov, T. R. [1 ]
Mardanova, A. M. [1 ]
Cheremin, A. M. [1 ]
Rudenskaya, G. N. [2 ]
Sharipova, M. R. [1 ]
机构：
[1] Kazan Fed Univ, Kazan 420008, Russia
[2] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119991, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2013年 / 39卷 / 01期
关键词：
glutamyl endopeptidase; B; pumilus; heterologous protein; isolation; different phases of the culture growth; ACID-SPECIFIC ENDOPEPTIDASE; MOLECULAR-CLONING; INTERMEDIUS; SUBTILIS; LICHENIFORMIS; PURIFICATION; SEQUENCE; PROTEINASE; PROTEASE; BINDING;
D O I：
10.1134/S1068162013010032
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Bacillus pumilus 3-19 Glutamyl peptidase (EC 3.4.21.19) was isolated from the culture medium of the B. subtilis recombinant strain at the following stages of the bacillus growth: a decelerating growth phase and a stationary growth phase. The action of the purified preparations of the enzyme on different phases of its growth was studied on the insulin B-chain and various protein and peptide substrates. Physicochemical properties of the enzyme were compared for different phases of its growth. The glutamyl endopeptidase preparations differed in their catalytic characteristics and their sensitivity to the metal cations.
引用
收藏
页码：40 / 47
页数：8
相关论文
共 50 条
  • [41] Growth conditions and production of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain
    Kirillova, YM
    Mikhailova, EO
    Balaban, NP
    Mardanova, AM
    Rudenskaya, GN
    Kostrov, SV
    Sharipova, MR
    MICROBIOLOGY, 2006, 75 (02) : 136 - 141
  • [42] Characterization of Thermostable Serine Alkaline Protease from an Alkaliphilic Strain Bacillus pumilus MCAS8 and Its Applications
    Jayakumar, Renganathan
    Jayashree, Shanmugam
    Annapurna, Balumuri
    Seshadri, Sundaram
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 168 (07) : 1849 - 1866
  • [43] Immobilization of Xylanase from Bacillus pumilus Strain MK001 and its Application in Production of Xylo-oligosaccharides
    Mukesh Kapoor
    Ramesh Chander Kuhad
    Applied Biochemistry and Biotechnology, 2007, 142 : 125 - 138
  • [44] Characterization of Thermostable Serine Alkaline Protease from an Alkaliphilic Strain Bacillus pumilus MCAS8 and Its Applications
    Renganathan Jayakumar
    Shanmugam Jayashree
    Balumuri Annapurna
    Sundaram Seshadri
    Applied Biochemistry and Biotechnology, 2012, 168 : 1849 - 1866
  • [45] RNA sequencing reveals small RNAs in Bacillus pumilus under different growth phases of the protease fermentation process
    Xu, Yunfan
    Zhao, Xianxian
    He, Tingting
    Huang, Yu
    Li, Xi
    Qin, Jia
    Song, Ting
    Wang, Haiyan
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 104 (02) : 833 - 852
  • [46] RNA sequencing reveals small RNAs in Bacillus pumilus under different growth phases of the protease fermentation process
    Yunfan Xu
    Xianxian Zhao
    Tingting He
    Yu Huang
    Xi Li
    Jia Qin
    Ting Song
    Haiyan Wang
    Applied Microbiology and Biotechnology, 2020, 104 : 833 - 852
  • [47] Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases
    Li, Xiaohui
    Ding, Xuezhi
    Xia, Liqiu
    Sun, Yunjun
    Yuan, Can
    Yin, Jia
    SCIENTIFIC WORLD JOURNAL, 2012,
  • [48] PURIFICATION AND PROPERTIES OF AN EXOCELLULAR GAMMA-GLUTAMYL-TRANSFERASE ARYLAMIDASE FROM BACILLUS SP STRAIN NO-12
    HWANG, SY
    OISHI, K
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, 49 (11): : 3255 - 3264
  • [49] In-Vitro Refolding and Characterization of Recombinant Laccase (CotA) From Bacillus pumilus MK001 and Its Potential for Phenolics Degradation
    Sandeep Kumar
    Kavish Kr. Jain
    Shikha Rani
    Kailash N. Bhardwaj
    Manisha Goel
    Ramesh Chander Kuhad
    Molecular Biotechnology, 2016, 58 : 789 - 800
  • [50] UPTAKE OF PHOSPHORUS BY MAIZE AT 3 DIFFERENT STAGES OF ITS GROWTH
    SINGH, S
    VERMA, MM
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES INDIA SECTION A-PHYSICAL SCIENCES, 1967, 37 : 391 - &
12345