High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis

被引：10
|
作者：
Gabriel, Emma M. [1 ]
Douarre, Pierre E. [1 ]
Fitzgibbon, Siobhan [2 ]
Clair, James [2 ]
Lucey, Brigid [1 ,3 ]
Coffey, Aidan [1 ]
O'Mahony, Jim M. [1 ]
机构：
[1] Cork Inst Technol, Cork, Ireland
[2] Mercy Univ Hosp, Cork, Ireland
[3] Cork Univ Hosp, Cork, Ireland
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 90卷 / 02期
关键词：
High resolution melting; Staphylococcus epidermidis; Linezolid resistance; G2576T mutation; MECHANISMS;
D O I：
10.1016/j.mimet.2012.04.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a novel HRM-PCR (high-resolution melting) assay capable of the accurate identification of the G2576T point mutation in domain V of the 235 rRNA genes attributed to linezolid resistance in Staphylococcus epidermidis. This rapid method demonstrated 100% correlation with the previously established NheI restriction digest assay. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：134 / 136
页数：3
相关论文
共 50 条
  • [41] Rapid detection of Echinococcus species by a high-resolution melting (HRM) approach
    Santos, Guilherme Brzoskowski
    Espinola, Sergio Martin
    Ferreira, Henrique Bunselmeyer
    Margis, Rogerio
    Zaha, Arnaldo
    PARASITES & VECTORS, 2013, 6
  • [42] Rapid detection of Echinococcus species by a high-resolution melting (HRM) approach
    Guilherme Brzoskowski Santos
    Sergio Martín Espínola
    Henrique Bunselmeyer Ferreira
    Rogerio Margis
    Arnaldo Zaha
    Parasites & Vectors, 6
  • [43] High-resolution melting curve analysis, a rapid and affordable method for mutation analysis in childhood acute myeloid leukemia
    Liu, Yin
    Tang, Jingyan
    Wakamatsu, Peter
    Xue, Huiliang
    Chen, Jing
    Gaynon, Paul S.
    Shen, Shuhong
    Sun, Weili
    FRONTIERS IN PEDIATRICS, 2014, 2
  • [44] Rapid and reliable detection of LINE-1 hypomethylation using high-resolution melting analysis
    Stanzer, Stefanie
    Balic, Marija
    Strutz, Jasmin
    Heitzer, Ellen
    Obermair, Florian
    Hauser-Kronberger, Cornelia
    Samonigg, Hellmut
    Dandachi, Nadia
    CLINICAL BIOCHEMISTRY, 2010, 43 (18) : 1443 - 1448
  • [45] Rapid and sensitive detection of CALR exon 9 mutations using high-resolution melting analysis
    Lim, Ken-Hong
    Lin, Huan-Chau
    Chen, Caleb Gon-Shen
    Wang, Wei-Ting
    Chang, Yu-Cheng
    Chiang, Yi-Hao
    Lin, Ching-Sung
    Su, Nai-Wen
    Su, Ying-Wen
    Lin, Johnson
    Chang, Yi-Fang
    Chang, Ming-Chih
    Hsieh, Ruey-Kuen
    Kuo, Yuan-Yeh
    Chou, Wen-Chien
    CLINICA CHIMICA ACTA, 2015, 440 : 133 - 139
  • [46] Rapid and high-resolution detection of IgH gene rearrangements using PCR and melting curve analysis
    Uemura, A.
    Mori, S.
    Sugahara, K.
    Akamatsu, N.
    Tsuruda, K.
    Tsukasaki, K.
    Hirakata, Y.
    Atogami, S.
    Hasegawa, H.
    Yamada, Y.
    Kamihira, Shimeru
    INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, 2007, 29 (03) : 200 - 207
  • [47] Rapid detection and identification of Theileria equi and Babesia caballi by high-resolution melting (HRM) analysis
    Salim, Bashir
    Bakheit, Mohammed Ahmed
    Sugimoto, Chihiro
    PARASITOLOGY RESEARCH, 2013, 112 (11) : 3883 - 3886
  • [48] A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci
    Ozbak, Hani Adnan
    ANNALS OF SAUDI MEDICINE, 2018, 38 (03) : 200 - 207
  • [49] Rapid detection and identification of Theileria equi and Babesia caballi by high-resolution melting (HRM) analysis
    Bashir Salim
    Mohammed Ahmed Bakheit
    Chihiro Sugimoto
    Parasitology Research, 2013, 112 : 3883 - 3886
  • [50] Rapid Detection and Identification of Nontuberculous Mycobacterial Pathogens in Fish by Using High-Resolution Melting Analysis
    Thu Nguyet Phung
    Caruso, Domenico
    Godreuil, Sylvain
    Keck, Nicolas
    Vallaeys, Tatiana
    Avarre, Jean-Christophe
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (24) : 7837 - 7845
12345