Optical Evaluation of Effects of Energy Substrates on PHB Accumulation for Bioplastic Production

被引：1

作者：

Stasczak, Alicja ^{[1
]}

Langer-Maciol, Hanna ^{[1
]}

Widzisz, Karolina ^{[1
]}

Sliwinska, Wiktoria ^{[1
]}

Lucinska, Kinga ^{[1
]}

Wencel, Przemyslaw ^{[1
]}

Strozik, Barbara ^{[1
]}

Frackiewicz, Mariusz ^{[2
]}

Skupin, Piotr ^{[1
]}

Choinski, Dariusz ^{[1
]}

Student, Sebastian ^{[3
]}

机构：

[1] Silesian Tech Univ, Dept Automat Control & Robot, Akad 16, PL-44100 Gliwice, Poland

[2] Silesian Tech Univ, Dept Data Sci & Engn, Akad 16, PL-44100 Gliwice, Poland

[3] Silesian Tech Univ, Biotechnol Ctr, Krzywoustego 8, PL-44100 Gliwice, Poland

来源：

ENERGIES | 2022年 / 15卷 / 22期

关键词：

polyhydroxybutyrate (PHB); polyhydroxyalkanoates (PHA); bioplastic; Pseudomonas fluorescens; PSEUDOMONAS-FLUORESCENS;

D O I：

10.3390/en15228390

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

To date, hundreds of millions tons of plastics has been produced worldwide. Their production and disposal are associated with high pollution and carbon release into the atmosphere. A more environmentally friendly alternative is bioplastics, and the most popular is polyhydroxybutyrate (PHB) polymer. Large amounts of PHB can be obtained from activated sludge where used cooking oil or other industrial waste can be used as potential substrates. In this work, efficient bioplastic production strategies are studied, and the considered substrate is a mixture of oil and peptone. Pseudomonas fluorescens bacteria are used to accumulate PHB, and the cultivation of microorganisms is carried out in batch and continuous-flow bioreactors. Microscopic observations and laboratory essays are performed to confirm presence of PHB and other key parameters. The obtained results allow us to determine the optimal feeding strategy.

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页数：16

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