Cell proliferation, viability, and in vitro differentiation of equine mesenchymal stem cells seeded on bacterial cellulose hydrogel scaffolds

被引:78
|
作者
Favi, Pelagie M. [1 ]
Benson, Roberto S. [1 ]
Neilsen, Nancy R. [2 ]
Hammonds, Ryan L. [1 ]
Bates, Cassandra C. [3 ]
Stephens, Christopher P. [4 ,5 ]
Dhar, Madhu S. [3 ]
机构
[1] Univ Tennessee, Dept Mat Sci & Engn, Coll Engn, Knoxville, TN 37996 USA
[2] Univ Tennessee, Dept Biomed & Diagnost Sci, Coll Vet Med, Knoxville, TN 37996 USA
[3] Univ Tennessee, Dept Large Anim Clin Sci, Coll Vet Med, Knoxville, TN 37996 USA
[4] Univ Tennessee, Grad Sch Med, Dept Surg, Knoxville, TN 37996 USA
[5] Univ Tennessee, Ctr Mat Proc, Knoxville, TN 37996 USA
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2013年 / 33卷 / 04期
关键词
Mesenchymal stem cell; Bacterial cellulose; Tissue engineering; Bone; Cartilage; MARROW STROMAL CELLS; CALCIUM-DEFICIENT HYDROXYAPATITE; BONE-MARROW; CHONDROGENIC DIFFERENTIATION; MECHANICAL-PROPERTIES; AGAROSE; COLLAGEN; DESIGN;
D O I
10.1016/j.msec.2012.12.100
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
The culture of multipotent mesenchymal stem cells on natural biopolymers holds great promise for treatments of connective tissue disorders such as osteoarthritis. The safety and performance of such therapies relies on the systematic in vitro evaluation of the developed stem cell-biomaterial constructs prior to in vivo implantation. This study evaluates bacterial cellulose (BC), a biocompatible natural polymer, as a scaffold for equine-derived bone marrow mesenchymal stem cells (EqMSCs) for application in bone and cartilage tissue engineering. An equine model was chosen due to similarities in size, load and types of joint injuries suffered by horses and humans. Lyophilized and critical point dried BC hydrogel scaffolds were characterized using scanning electron microscopy (SEM) to confirm nanostructure morphology which demonstrated that critical point drying induces fibre bundling unlike lyophilisation. EqMSCs positively expressed the undifferentiated pluripotent mesenchymal stem cell surface markers CD44 and CD90. The BC scaffolds were shown to be cytocompatible, supporting cellular adhesion and proliferation, and allowed for osteogenic and chondrogenic differentiation of EqMSCs. The cells seeded on the BC hydrogel were shown to be viable and metabolically active. These findings demonstrate that the combination of a BC hydrogel and EqMSCs are promising constructs for musculoskeletal tissue engineering applications. Published by Elsevier B.V.
引用
收藏
页码:1935 / 1944
页数:10
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