Structural and functional chromatin organization of the SUP35 gene in Saccharomyces cerevisiae yeast

被引：0
|
作者：
Ryabinkova, NA [1 ]
Vodopyanova, LG [1 ]
Samsonova, MG [1 ]
Myasnikova, EM [1 ]
Osipova, TN [1 ]
机构：
[1] ST PETERSBURG STATE TECH UNIV,DEPT APPL MATH,ST PETERSBURG 199064,RUSSIA
来源：
GENETIKA | 1997年 / 33卷 / 04期
关键词：
D O I：
暂无
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Structural and functional organization of the 5' region of the SUP35 gene was analyzed in Saccharomyces cerevisiae yeast. Indirect DNA end labeling allowed two nuclease-hypersensitive sites and a region involved in nucleosomes to be revealed. DNase I and micrococcal nuclease hypersensitive sites were localized to almost the same regions: -461 ... -372 bp and -271 ... -91 bp for DNase I and -461 ... -356 bp and -231 ... -79 for micrococcal nuclease. Nucleosomes were localized to a region +22 ... +339 bp. Both the location of DNase I and micrococcal nuclease hypersensitive sites within the promoter region and the location of nucleosomes within the coding region remain the same at different cell culture growth phases. However, positioning nucleosomes were revealed within the SUP35 coding region only at the late logarithmic phase; the radioautographic pattern of them does not depend on the extent of nuclease digestion.
引用
收藏
页码：451 / 457
页数：7
相关论文
共 50 条
  • [41] Increased [PSI+] Appearance by Fusion of Rnq1 with the Prion Domain of Sup35 in Saccharomyces cerevisiae
    Choe, Young-Jun
    Ryu, Yangkyun
    Kim, Hyun-Jin
    Seok, Yeong-Jae
    EUKARYOTIC CELL, 2009, 8 (07) : 968 - 976
  • [42] DELETION ANALYSIS OF THE SUP2 GENE OF THE YEAST SACCHAROMYCES-CEREVISIAE
    KUSHNIROV, VV
    TERAVANESYAN, MD
    DAGKESAMANSKAYA, AR
    CHERNOV, YO
    INGEVECHTOMOV, SG
    SMIRNOV, VN
    MOLECULAR BIOLOGY, 1990, 24 (04) : 836 - 840
  • [43] Targeted mutagenesis of the oligopeptide repeat domain of the yeast prion Sup35
    Knox, James
    Davis, Emily
    MacLea, Kyle
    FASEB JOURNAL, 2014, 28 (01):
  • [44] Whole genome sequencing data and analyses of the underlying SUP35 transcriptional regulation for a Saccharomyces cerevisiae nonsense suppressor mutant
    Matveenko, Andrew G.
    Drozdova, Polina B.
    Moskalenko, Svetlana E.
    Tarasov, Oleg V.
    Zhouravleva, Galina A.
    DATA IN BRIEF, 2019, 23
  • [45] Origins and kinetic consequences of diversity in Sup35 yeast prion fibers
    Angela H. DePace
    Jonathan S. Weissman
    Nature Structural Biology, 2002, 9 : 389 - 396
  • [46] Suppression of polyglutamine toxicity by the yeast sup35 prion domain in Drosophila
    Li, Ling-Bo
    Xu, Kexiang
    Bonini, Nancy M.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (52) : 37694 - 37701
  • [47] Origins and kinetic consequences of diversity in Sup35 yeast prion fibers
    DePace, AH
    Weissman, JS
    NATURE STRUCTURAL BIOLOGY, 2002, 9 (05) : 389 - 396
  • [48] LOCALIZATION OF POSSIBLE FUNCTIONAL DOMAINS IN SUP2 GENE-PRODUCT OF THE YEAST SACCHAROMYCES-CEREVISIAE
    KUSHNIROV, VV
    TERAVANESYAN, MD
    SURGUCHOV, AP
    SMIRNOV, VN
    INGEVECHTOMOV, SG
    FEBS LETTERS, 1987, 215 (02) : 257 - 260
  • [49] THE YEAST SUP35 PROTEIN FORMS A LARGE NUMBER OF INFECTIOUS STRUCTURES
    Huang, Yu-Wen
    King, Chih-Yen
    PROTEIN SCIENCE, 2019, 28 : 156 - 156
  • [50] Interactions of [NSI+] prion-like determinant with SUP35 and VTS1 genes in Saccharomyces cerevisiae
    A. A. Nizhnikov
    A. M. Kondrashkina
    A. P. Galkin
    Russian Journal of Genetics, 2013, 49 : 1004 - 1012
12345