Determination of Analytical Wavelength in Derivative Spectrophotometry

The work presents two methods for preliminary determination of analytical wavelength when a binary system is analysed by derivative spectrophotometry. The method of disturbing effect involves the recording of derivative spectra of a series of two-component solutions where the concentration of one component of the mixture remains unchanged, whereas the concentration of the other changes. The method of sixteen solutions involves the recording of derivative spectra of a series of 16 solutions, in which to four concentrations of the one component correspond four different concentrations of the other component of the mixture.