Comparative Analysis of Fiber Morphogenesis Genes of Calotropis procera and Gossypium hirsutum

被引:0
|
作者
Khatoon, Asia [1 ]
Iqbal, Nadia [1 ]
Asif, Muhammad [1 ]
Cheema, Hafiza Masooma Naseer [2 ]
Saeed, Muhammad [1 ]
Bashir, Aftab [3 ]
机构
[1] NIBGE, Faisalabad, Pakistan
[2] Univ Agr Faisalabad, Dept Plant Breeding & Genet, Plant Genet Resources Lab, Faisalabad, Pakistan
[3] FC Coll Univ, Dept Biol Sci, Lahore, Pakistan
关键词
Transcript profiling; Cotton fiber; Fiber genes; EST's; Transcript analysis; PLASMA-MEMBRANE AQUAPORINS; COTTON FIBER; SUCROSE SYNTHASE; CELL ELONGATION; EXPRESSION; WATER; WALL; IDENTIFICATION; CHANNELS;
D O I
10.17957/IJAB/15.0492
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Cotton (Gossypium spp.) is the leading fiber crop in the world. The earnings from fiber lint are associated with its quality traits. For studying the major fiber development specific genes, cDNA libraries were used to establish 1000 EST's (expressed sequence tags), each from the fast-growing fibers of Calotropis procera and Gossypium hirsutum L. Most abundantly expressed transcripts of C. procera coded for expansins, aquaporins and sugar/salt transporters. Four homologs of expansin gene (CpEXPA1, CpEXPA2, CpEXPA3 and CpEXPA4) and four of aquaporins (CpTiP1, CpTiP2, CpPiP1 and CpPiP2) were found to be the most abundantly expressed transcripts in fast growing C. procera fiber cells. Cotton fiber EST's revealed two homologs of expansin, one for G. hirsutum plasma membrane intrinsic protein (GhPiP1) and one transcript for sucrose synthase. The GhPiP1 had 70% amino acid identity to CpPiP1, while no homolog was detected for CpPiP2 in cotton fibers. Real-time PCR data showed that CpPiP1 had about 40% higher transcript level of the gene in C. procera as compared to cotton. CpPiP2 showed high level of transcripts in C. procera fiber only. On the other hand, CpEXPA3 was the closest homologue of GhEXP15 (76% amino acid identity) and its transcript level was 80% higher in C. procera fibers. Expression of sucrose synthase was at its high level in 5-15 DPA cotton fibers. These studies demonstrated that CpPiP2, CpEXPA3 and sucrose synthase are the best candidate genes for fiber modification in G. hirsutum. (C) 2018 Friends Science Publishers
引用
收藏
页码:288 / 296
页数:9
相关论文
共 50 条
  • [21] Transcriptome analysis reveals genes potentially related to high fiber strength in a Gossypium hirsutum line IL9 with Gossypium mustelinum introgression
    Chen, Qi
    Wang, Wei
    Khanal, Sameer
    Han, Jinlei
    Zhang, Mi
    Chen, Yan
    Li, Zhenjiang
    Wang, Kai
    Paterson, Andrew H.
    Yu, Jiwen
    Chee, Peng W.
    Wang, Baohua
    GENOME, 2021, 64 (11) : 985 - 995
  • [22] Transformation and inheritance of Bt genes in Gossypium hirsutum
    Rashid, Bushra
    Saleem, Zafar
    Husnain, Tayyab
    Riazuddin, Sheikh
    JOURNAL OF PLANT BIOLOGY, 2008, 51 (04) : 248 - 254
  • [23] Identification of salt responsive genes using comparative microarray analysis in Upland cotton (Gossypium hirsutum L.)
    Rodriguez-Uribe, Laura
    Higbie, Sarah M.
    Stewart, James McD.
    Wilkins, Thea
    Lindemann, W.
    Sengupta-Gopalan, C.
    Zhang, Jinfa
    PLANT SCIENCE, 2011, 180 (03) : 461 - 469
  • [24] Comparative proteomic analysis of seed germination between allotetraploid cotton Gossypium hirsutum and Gossypium barbadense
    Huwanixi, Aishuake
    Peng, Zhao
    Li, Shenglan
    Zhou, Yutian
    Zhao, Sixian
    Wan, Cuihong
    JOURNAL OF PROTEOMICS, 2024, 297
  • [25] Transcriptome analysis combined with metabolome analysis reveals the significant functions of CesA genes in cotton (Gossypium hirsutum) fiber length development
    CUI, Z. H. E. N. K. U. I.
    SUN, G. U. I. Q. I. N.
    ZHAO, Q. U. A. N. Z. H., I
    BIOCELL, 2022, 46 (09) : 2133 - 2144
  • [26] CLOTH FROM BAST FIBER OF THE CALOTROPIS-PROCERA (AAK) PLANT
    VARSHNEY, AC
    BHOI, KL
    BIOLOGICAL WASTES, 1988, 26 (03): : 229 - 232
  • [27] CHEMICAL-ANALYSIS OF CALOTROPIS-PROCERA LATEX
    PANT, R
    CHATURVEDI, K
    CURRENT SCIENCE, 1989, 58 (13): : 740 - 742
  • [28] Cloning and expression analysis of novel Aux/IAA family genes in Gossypium hirsutum
    Han, Xiaoyong
    Xu, Xiaoyang
    Fang, David D.
    Zhang, Tianzhen
    Guo, Wangzhen
    GENE, 2012, 503 (01) : 83 - 91
  • [29] Phytochemical analysis of Calotropis procera with antimicrobial activity investigation
    Morsy, Nagy
    Al Sherif, Emad A.
    Abdel-Rassol, Tayseer M. A.
    MAIN GROUP CHEMISTRY, 2016, 15 (03) : 267 - 273
  • [30] Genome-wide identification and expression analysis of the DnaJ genes in Gossypium hirsutum
    Chen, Pengyun
    Xiao, Xianghui
    Li, Yunqing
    Gu, Lijiao
    Zhang, Yanli
    Peng, Yanchun
    Han, Xiao
    Wei, Fei
    INDUSTRIAL CROPS AND PRODUCTS, 2025, 228