An ELISA Chip Based on an EWOD Microfluidic Platform

被引：16

作者：

Zhu, Liang ^{[1
]}

Feng, Yanying ^{[1
]}

Ye, Xiongying ^{[1
]}

Feng, Jinyang ^{[1
]}

Wu, Yanbin ^{[1
]}

Zhou, Zhaoying ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Precis Instruments & Mechanol, State Key Lab Precis Measurement Technol & Instru, Beijing 100084, Peoples R China

来源：

JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY | 2012年 / 26卷 / 12-17期

基金：

中国国家自然科学基金;

关键词：

EWOD; droplet; lab-on-a-chip; ELISA; immunoassay; ELECTROWETTING-BASED ACTUATION; MERGING LIQUID DROPLETS; ON-A-CHIP; CIRCUITS;

D O I：

10.1163/156856111X600172

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

We have developed an enzyme-linked immunosorbent assay (ELISA) chip based on a digital microfluidic platform, in which droplet generation and manipulation are implemented using the electrowetting on dielectrics (EWOD) effect. First, droplet transportability was experimentally tested on silicon test chips with different control electrodes for optimizing the EWOD-based droplet manipulation, and then tested on transparent indium-tin-oxide (ITO) chips. The latter were then used for the development of an ELISA chip, on which we carried out immunoassay with rat immunoglobulin G (IgG) and goat anti-rat IgG marked with horseradish peroxidase as an example. In driving the reagent solutions, the average velocity of droplets is up to 5-7 mm/s on the ELISA chip, and a chip-scaled immunoassay can be finished within 20 min by using colorimetric detection, with the volume of the sample and reagents of only 0.5-1 mu l. (C) Koninklijke Brill NV, Leiden, 2011

引用

页码：2113 / 2124

页数：12

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