DNA Interaction Studies of an Antiviral Drug, Ribavirin, Using Different Instrumental Methods

被引：15
|
作者：
Shahabadi, Nahid [1 ]
Kalar, Zeinab Mirzaei [1 ]
Rayegani, Asad Veysi [2 ]
机构：
[1] Razi Univ, Dept Chem, Fac Sci, Kermanshah 74155, Iran
[2] Med Sci Univ Kermanshah, Dept Biochem, Kermanshah, Iran
来源：
DNA AND CELL BIOLOGY | 2012年 / 31卷 / 05期
关键词：
MIXED-POLYPYRIDYL COMPLEXES; RESPIRATORY SYNCYTIAL VIRUS; CHRONIC HEPATITIS-C; HUMAN SERUM-ALBUMIN; CRYSTAL-STRUCTURE; FLUORESCENCE SPECTROSCOPY; BINDING PROPERTIES; CLEAVAGE; COBALT(III); COPPER(II);
D O I：
10.1089/dna.2011.1456
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Interaction of ribavirin with CT-DNA was investigated by emission, absorption, circular dichroism, and viscosity studies to determine the binding mode and binding constant of this drug with DNA. The calculated binding constant, K-b, obtained from UV-vis absorption studies was 4.6 x 10(3) M-1. In fluorimetric studies, the enthalpy (Delta H<0) and entropy (Delta S>0) of the reaction between ribavirin and CT-DNA showed a hydrophobic interaction. In addition, in the circular dichroism spectrum, the drug induces a B -> A structural transition of CT-DNA. These results demonstrate that ribavirin interacts with CT-DNA via the groove binding mode. It was observed that the drug has ability to cleave supercoiled plasmid DNA.
引用
收藏
页码：876 / 882
页数：7
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