An Acousto-Optic Hyperspectral Unit for Histological Study of Microscopic Objects

被引：8
|
作者：
Polschikova, O. V. [1 ]
Machikhin, A. S. [1 ,2 ]
Ramazanova, A. G. [1 ]
Bratchenko, I. A. [3 ]
Pozhar, V. E. [1 ]
Danilycheva, I. V. [4 ,5 ]
Katunina, O. R. [6 ]
Danilychev, M. V. [7 ]
机构：
[1] Russian Acad Sci, Sci & Technol Ctr Unique Instrumentat, Moscow 117342, Russia
[2] Tech Univ, Natl Res Univ, Moscow Power Engn Inst, Moscow 111250, Russia
[3] Natl Res Univ, Korolyov Samara State Aerosp Univ, Samara 443086, Russia
[4] Fed Med Biol Agcy Russia, Inst Immunol, Natl Res Ctr, Moscow 115478, Russia
[5] Evdokimov Moscow State Univ Med & Dent, Moscow 127473, Russia
[6] Burnasyan Fed Med Biophys Ctr, State Res Ctr, Moscow 123098, Russia
[7] Russian Acad Sci, Kotelnikov Inst Radio Engn & Elect, Moscow 125009, Russia
来源：
OPTICS AND SPECTROSCOPY | 2018年 / 125卷 / 06期
基金：
俄罗斯基础研究基金会;
关键词：
SKIN;
D O I：
10.1134/S0030400X19020188
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The problem of the development of an adjustable video-spectrometer unit based on an acousto-optic tunable filter has been considered. This unit is embedded into an optical system of microscopes for hyperspectral analysis of microscopic objects. For this purpose, light-energy conjugation of the filter with microscopes is performed. We have calculated the conjugating optical system, which yields an equality of field of view for a wideband and spectral channel. The experimental model of the developed device has been described. Examples of detection for test objects and histological sections along with the calculation results of the spectral parameters for various skin tissues are given. The developed video-spectrometer unit considerably extends the functionality of microscopes and can be widely used to solve various problems of biomedical diagnostics.
引用
收藏
页码：1074 / 1080
页数：7
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