Effects of pH and sugar concentration in Zygosaccharomyces rouxii growth and time for spoilage in concentrated grape juice at isothermal and non-isothermal conditions

被引:22
|
作者
Rojo, M. C. [1 ,3 ]
Arroyo Lopez, F. N. [2 ]
Lerena, M. C. [1 ,3 ]
Mercado, L. [3 ]
Torres, A. [1 ,4 ]
Combina, M. [1 ,3 ]
机构
[1] Consejo Nacl Invest Cient & Tecn CONICET, Buenos Aires, DF, Argentina
[2] Inst Grasa CSIC, Dept Food Biotechnol, Seville 41012, Spain
[3] Inst Nacl Tecnol Agr EM Mza INTA, Estac Expt Agr Mendoza, Wine Res Ctr, RA-5507 Mendoza, Argentina
[4] Univ Nacl Rio Cuarto, Fac Ciencias Exactas Fisicoquim & Nat, Microbiol & Inmunol Dept, Rio Cuarto, Argentina
关键词
Response surface methodology; Zygosaccharomyces rouxii; Concentrated grape juice; pH; Sugar concentration; Spoilage; BAKERY PRODUCTS; YEASTS; GLUCOSE; SORBATE; SUCROSE; MEDIA; MODEL;
D O I
10.1016/j.fm.2013.08.017
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of pH (1.7-3.2) and sugar concentration (64-68 degrees Brix) on the growth of Zygosaccharomyces rouxii MC9 using response surface methodology was studied. Experiments were carried out in concentrated grape juice inoculated with Z rouxii at isothermal conditions (23 degrees C) for 60 days. pH was the variable with the highest effect on growth parameters (potential maximum growth rate and lag phase duration), although the effect of sugar concentration were also significant. In a second experiment, the time for spoilage by this microorganism in concentrated grape juice was evaluated at isothermal (23 degrees C) and non-isothermal conditions, in an effort to reproduce standard storage and overseas shipping temperature conditions, respectively. Results show that pH was again the environmental factor with the highest impact on delaying the spoilage of the product. Thereby, a pH value below 2.0 was enough to increase the shelf life of the product for more than 60 days in both isothermal and non-isothermal conditions. The information obtained in the present work could be used by producers and buyers to predict the growth and time for spoilage of Z rouxii in concentrated grape juice. (C) 2013 Elsevier Ltd. All rights reserved.
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页码:143 / 150
页数:8
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