Isolation of a snake venom phospholipase A2 (PLA2) inhibitor (AIPLAI) from leaves of Azadirachta indica (Neem):: Mechanism of PLA2 inhibition by AIPLAI in vitro condition

被引：46
|
作者：
Mukherjee, Ashis K. [1 ]
Doley, Robin [1 ]
Saikia, Debashree [1 ]
机构：
[1] Tezpur Univ, Dept Mol Biol & Biotechnol, Tezpur 784028, Assam, India
来源：
TOXICON | 2008年 / 51卷 / 08期
关键词：
snake venom; cobra venom; viper venom; Neem; Azadirachta indica; phospholipase A(2)-inhibitor; non-competitive inhibition;
D O I：
10.1016/j.toxicon.2008.03.021
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A compound (AIPLAI (Azadirachta indica PLA(2) inhibitor)) purified from the methanolic leaf extract of A. indica (Neem) inhibits the cobra and Russell's viper venoms (RVVs) phospholipase A(2) enzymes in a dose-dependent manner. Inhibition of catalytic and tested pharmacological properties of cobra venom (Naja naja and Naja kaouthia) PLA(2) enzymes by AIPLAI is significantly higher (P<0.05) compared to the inhibition of PLA(2) enzymes of crude RVV (Daboia russelli) when tested under the same condition. Kinetic study reveals that in in vitro condition, AIPLAI inhibits the purified N. kaouthia PLA(2) enzymes in a non-competitive manner. The AIPLAI is quite stable at room temperature. The present study shows that AIPLAI holds good promise for the development of novel anti-snake venom drug in future. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1548 / 1553
页数：6
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