Development of Real-Time PCR Assay for Detection of Vibrio cholerae

被引：0
|
作者：
Bielawska-Drozd, Agata [1 ]
Mirski, Tomasz [1 ]
Bartoszcze, Michal [1 ]
Cieslik, Piotr [1 ]
Roszkowiak, Anna [1 ]
Michalski, Aleksander [1 ]
机构：
[1] Mil Inst Hyg & Epidemiol, Biol Threat Identificat & Countermeasure Ctr, PL-24100 Pulawy, Poland
来源：
POLISH JOURNAL OF ENVIRONMENTAL STUDIES | 2012年 / 21卷 / 02期
关键词：
real-time PCR; Vibrio cholerae; ompW; ompU; zot; tcpA; rfbO1; rfbO139; ctxA; ZONULA-OCCLUDENS TOXIN; POLYMERASE-CHAIN-REACTION; PATHOGENICITY ISLAND; WATER SAMPLES; GENES CTXA; DUPLEX-PCR; VIRULENCE; STRAINS; O1; NON-O1;
D O I：
暂无
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Vibrio cholerae is the etiological agent of epidemic cholera. Symptoms include stomach ache, bloody diarrhea, and vomiting, which lead to severe dehydration and even death. Environmental monitoring as well as rapid and accurate identification of this pathogen are important for public health protection. In this study, a real-time PCR method for the detection of toxigenic V cholerae was developed. In total, 63 environmental and clinical strains were tested for the presence of seven targets, namely ompW, ompU, tcpA, ctxA, zot, rfbO1, and rfbO139. The proposed method is specific, simple, and fast, and can be used for detection of toxigenic and non-toxigenic V cholerae strains. The minimum detection limit of this assay for V cholerae in environmental water samples was 1.4 CFU/ml.
引用
收藏
页码：279 / 288
页数：10
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