EAMUS™ -: Theoretical considerations and practical experiences with an Internet-based automated immunohistochemistry measurement system

被引:0
|
作者
Kayser, G [1 ]
Radzizowski, D [1 ]
Bzdyl, P [1 ]
Sommer, R [1 ]
Kayser, K [1 ]
机构
[1] Univ Hosp Freiburg, Inst Pathol, Freiburg, Germany
关键词
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暂无
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Aims: To develop and implement an automated image measurement system for immunohistochemically stained slides. Theoretical considerations: The system requires sophisticated segmentation algorithms, knowledge of artifacts; standardized measurements; active protocols, automated error detection. Methods: The implemented system is divided into a common server platform and a specific image quantification system. It includes stereological algorithms, object quantification, syntactic structure analysis, and quality assurance. Results: The system measures DAB, AP, and fluorescence images (FITC, etc.). It possesses a reproducibility and stability > 98%. Conclusions: Its home page is {http://www.pathology-online.org}.
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页码:291 / 296
页数:6
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