A novel electrochemical aptasensor for highly sensitive and quantitative detection of the streptomycin antibiotic

被引:46
|
作者
Ghanbari, Kazhal [1 ]
Roushani, Mahmoud [1 ]
机构
[1] Univ Ilam, Dept Chem, Ilam, Iran
关键词
Aptamer; Gold nanoparticle; Thiol graphene quantum dots; Streptomycin; GRAPHENE QUANTUM DOTS; HUMAN CHORIONIC-GONADOTROPIN; REDOX PROBE; NANOCOMPOSITE; NANOPARTICLES; IMMUNOSENSOR; LABEL; REDUCTION; OXIDATION; RESIDUES;
D O I
10.1016/j.bioelechem.2017.11.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the present study, we report a facile approach to employ gold nanoparticle (AuNPs) and thiol graphene quantum dots (GQD-SH) as the nanomaterial for ultrasensitive detection of streptomycin (STR). Based on this strategy, a GQD-SH was immobilized onto the surface of a glassy carbon electrode (GCE). AuNPs have been immobilized on SH groups of GQDs through bonding formation of Au-S and Apt have been loaded on the electrode surface through the interaction between thiol group of aptamer. By incubating STR as a target onto the surface of the prepared Apt/AuNPs/GQD-SH/GCE as a proposed nanoaptasensor, the Apt/STR complex was formed and the changes of the electrochemical signal were evaluated with the EIS technique. The proposed nanoaptasensor showed wide linear range from 0.1 to 700 pg ml(-1). Finally, the proposed nanoaptasensor was successfully applied for the determination of STR in real samples and satisfactory results were obtained. (C) 2017 Published by Elsevier B.V.
引用
收藏
页码:43 / 48
页数:6
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