Fluorescence and absorbance measurements for chromatographic analysis using a miniaturized micro-optical detection unit

被引：0
|
作者：
Van Overmeire, Sara [1 ]
Ottevaere, Heidi [1 ]
Desmet, Gert [2 ]
Thienpont, Hugo [1 ]
机构：
[1] Vrije Univ Brussel, Dept Appl Phys & Photon FirW TONA, B-1050 Brussels, Belgium
[2] Vrije Univ Brussel, Dept Chem Engn FirWCHIS, B-1050 Brussels, Belgium
来源：
BIOPHOTONICS: PHOTONIC SOLUTIONS FOR BETTER HEALTH CARE | 2008年 / 6991卷
关键词：
optics; fluorescence; absorbance; chromatography; plastics; rapid prototyping; replication;
D O I：
10.1117/12.780490
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
We present a micro-optical detection unit for both laser induced fluorescence and absorbance analysis in fused silica capillaries., which can be used for chromatographic applications. The detection system is designed by means of non-sequential ray tracing simulations and prototyped by means of Deep Proton Writing. Such a prototyped master component is afterwards replicated by means of elastomeric moulding and vacuum casting. In a proof-of-concept demonstration the prototyped master micro-optical unit is used for the detection of various concentrations of coumarin dyes. The detection limit (SNR=3.3) achieved measures 0.6nM for fluorescence analysis and 12 mu M for absorbance measurements in capillaries with an inner diameter of 150 mu m. We discuss the optimization of different measurement parameters of the detectors in the setup in order to achieve accurate, fast and sensitive measurements.
引用
收藏
页数：12
相关论文
共 50 条
  • [41] Implantable, insertable and wearable micro-optical devices for early detection of cancer
    Contag, Christopher H.
    2018 IEEE PHOTONICS CONFERENCE (IPC), 2018,
  • [42] Micro-optical force distribution sensing suitable for lump/artery detection
    Roozbeh Ahmadi
    Siamak Arbatani
    Muthukumaran Packirisamy
    Javad Dargahi
    Biomedical Microdevices, 2015, 17
  • [43] Design and analysis of a micro-optical position readout for acceleration sensing
    Shagam, RN
    Dickey, FM
    Holswade, SC
    MINIATURIZED SYSTEMS WITH MICRO-OPTICS AND MEMS, 1999, 3878 : 319 - 327
  • [44] Analysis of an encapsulated whispering gallery mode micro-optical sensor
    Nguyen, N. Q.
    Gupta, N.
    APPLIED PHYSICS B-LASERS AND OPTICS, 2009, 96 (04): : 793 - 801
  • [45] Replicating micro-optical structures using soft embossing technique
    Shang, Xiaobing
    Tan, Jin-Yi
    De Smet, Jelle
    Joshi, Pankaj
    Islamaj, Esma
    Cuypers, Dieter
    Vervaeke, Michael
    Van Erps, Jurgen
    Thienpont, Hugo
    De Smet, Herbert
    30TH EUROPEAN MASK AND LITHOGRAPHY CONFERENCE, 2014, 9231
  • [46] Fabrication of Infrared Micro-Optical Components Using Femtosecond Laser
    Niu Yahua
    Sun Shengzhi
    Liu Xiaofeng
    Qiu Jianrong
    LASER & OPTOELECTRONICS PROGRESS, 2023, 60 (23)
  • [47] Miniaturized Absorbance Based Cell Analysis System with Integrated Microfluidic and Optical Elements
    Rosenauer, Michael
    Vellekoop, Michiel J.
    2009 IEEE SENSORS, VOLS 1-3, 2009, : 1567 - 1570
  • [48] Actuated Micro-optical Submount Using a Dielectric Elastomer Actuator
    Jordan, G.
    McCarthy, D. N.
    Schlepple, N.
    Krissler, J.
    Schroeder, H.
    Kofod, G.
    IEEE-ASME TRANSACTIONS ON MECHATRONICS, 2011, 16 (01) : 98 - 102
  • [49] Fabrication of micro-optical components using femtosecond oscillator pulses
    Rodrigues, Vanessa R. M.
    Ramachandran, Hema
    Chidangil, Santhosh
    Mathur, Deepak
    FIFTH INTERNATIONAL CONFERENCE ON OPTICAL AND PHOTONICS ENGINEERING, 2017, 10449
  • [50] Analysis of an encapsulated whispering gallery mode micro-optical sensor
    N. Q. Nguyen
    N. Gupta
    Applied Physics B, 2009, 96
12345