Applications of Luminous Bacteria Enzymes in Toxicology

被引：45
|
作者：
Kratasyuk, Valentina A. [1 ,2 ]
Esimbekova, Elena N. [1 ,2 ]
机构：
[1] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[2] Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia
来源：
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2015年 / 18卷 / 10期
基金：
俄罗斯科学基金会;
关键词：
Bioluminescence; bioluminescent toxicity enzymatic assay; immobilization of enzymes; luciferase; total toxicity; HUMIC SUBSTANCES; DETOXIFICATION PROCESSES; BIOLUMINESCENT ASSAY; LUCIFERASE; SYSTEM; STABILIZERS; INHIBITION; TOXICITY; QUINONES; STARCH;
D O I：
10.2174/1386207318666150917100257
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH: FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.
引用
收藏
页码：952 / 959
页数：8
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