LIFETIME ESTIMATION OF MOVING VESICLES IN FREQUENCY-DOMAIN FLUORESCENCE LIFETIME IMAGING MICROSCOPY

被引：0

作者：

Roudot, Philippe ^{[1
]}

Kervrann, Charles ^{[1
]}

Waharte, Francois ^{[2
]}

机构：

[1] Inria Rennes Bretagne Atlantique, F-35042 Rennes, France

[2] PICT IBiSA, Inst Curie, CNRS, UMR 144, Paris, France

来源：

2012 9TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI) | 2012年

关键词：

FLIM; frequency-domain; phase modulation; spot tracking; template matching; M-estimation; TRACKING;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We propose a robust statistical framework for correcting the movements of vesicles in frequency domain FLIM imaging. Movement and lifetime are jointly estimated in a three-step procedure. Robust M-estimators are mainly used to improve accuracy in temporally-varying noisy images. The performance of the proposed method is demonstrated on both simulated and real samples.

引用

页码：668 / 671

页数：4

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